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脂联素对2型糖尿病大鼠主动脉内皮功能的影响

来自:中国糖尿病杂志  编辑:赵建刚 黄娜 侯宁宁 韩芳 孙晓东|点击数:|2015-03-12

  ·糖尿病基础研究·

  【摘要】 目的 观察APN对T2DM大鼠主动脉内皮功能的影响。 方法 采用高脂饮食加腹腔注射链脲佐菌素建立T2DM大鼠模型。造模成功后随机分为DM组9只和APN组8只,另设正常对照(NC)组10只。APN组给予尾静脉注射腺病毒重组APN,DM组和NC组给予同等剂量生理盐水注射。测定各组胸主动脉内皮功能,核因子-κB(NF-κB)表达及一氧化氮(NO)水平。 结果 与DM组相比,APN组高敏C反应蛋白(hsC-RP)[(1.85±0.56) vs (3.13±0.38) mg/L]和丙二醛[(2.44±0.28) vs (4.80±0.59) mg/L]降低,APN水平[(273.50±21.20) vs (94.97±25.12) μg/ L]升高(P<0.01)。NC组主动脉环最大舒张率高于其他两组[(81.7±5.2)% vs (65.9±9.9)% vs (36.0±8.9)%,P<0.05]。DM组主动脉NF-κB表达高于APN组和NC组,且APN组表达高于NC组(P<0.05)。NC组器官池中NO水平高于DM组和APN组[(125.6±17.7) vs (103.5±20.3) vs (72.5±9.1) μmol/g, P<0.05]。 结论 APN能改善T2DM大鼠主动脉内皮功能,其机理可能通过减少氧化应激(OS)和炎症反应,增加NO的产生有关。

  【关键词】 脂联素;内皮功能;糖尿病,2型

  【Abstract】 Objective To investigate the protective effect of adiponectin on vascular endothelial dysfunction in streptozotocin induced type 2 diabetic rat. Methods Diabetic rats were induced by high fat diet and intraperitoneal injection of a low dose of STZ. After the model was established successfully, a total of 17 diabetic rats were randomly divided into DM group (n=9) and APN group (n=8). 10 normal rats were selected as control group (NC). APN group was given intravenous injection of recombinant adenovirus adiponectin with 0.71 ×109 IU in each rat. The same dose of saline was injected in DM group and NC group. The endothelial function, nuclear factor-kappa B(NF-κB) expression and nitric oxide (NO) levels of thoracic aortas were measured. Results Compared with the diabetic rats, serum high sensitive C-reactive protein[(1.85±0.56) vs (3.13±0.38) mg/L] and malondialdehyde levels[(2.44±0.28) vs (4.80±0.59) mg/L] were significantly reduced, and serum adiponectin levels[(273.50±21.20) vs (94.97±25.12) μg/ L] were increased in APN group (P<0.01). The maximum relaxation rate of aortic ring was higher in NC group than that in APN group or DM group [(81.7±5.2)% vs (65.9±9.9)% vs (36.0±8.9)%,P<0.05]. The resuts of immunohistochemistry showed that the NF-κB expression in aorta was significantly higher in DM group than that in NC group, and was decreased after treatment with adiponectin. Compared with NC group, NO level was significantly lower in diabetic rats, and was significantly increased after treatment with adiponectin[(125.6±17.7) vs (103.5±20.3) vs (72.5±9.1) μmol/g, P<0.05]. Conclusions Adiponectin could protect the vascular endothelial function in type 2 diabetic rats. The mechanism may be explained by inhibiting oxidative stress, reducing inflammation, and increasing NO production.

  【Key words】 Adiponectin; Endothelial function; Diabetic mellitus, type 2

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