糖尿病基础研究

　　【摘要】目的 探讨增强Zeste同源2 (EZH2)与年龄相关性胰岛细胞功能的关系。方法 健康SD大鼠分为1、6、12及24月龄组，每组各6只。取大鼠胰腺，行免疫组化染色。Western blot检测胰岛内EZH2及胰岛素水平。行Ki67染色及TUNEL染色分别检测胰岛细胞增殖及凋亡。结果 随年龄增加，胰岛内EZH2表达逐渐减少：1月龄组(0.22516±0.03091);6月龄组(0.18427±0.02315);12月龄组(0.03165±0.01675);24月龄组0(P<0.01)。胰岛细胞增殖逐渐减少：6月龄组(0.36±0.03)%;12月龄组(0.61±0.02)%;24月龄组(0.12±0.02)%(P<0.01)。胰岛细胞凋亡逐渐增加：12月龄组(0.02±0.03)%;24月龄组(0.09±0.04)%(P<0.01)。胰岛内胰岛素水平：1月龄组(206.5±27.8);6月龄组(267.4±25.3);12月龄组(376.2±31.9);24月龄组(187.8±25.1)(P<0.01)。结论 随着年龄的增加，胰岛细胞逐渐衰老，胰岛细胞内EZH2表达逐渐减少，伴随着胰岛细胞增殖能力下降，凋亡增加，胰岛素水平逐渐减低。

　　[关键词]增强Zeste同源2;胰岛细胞增殖;凋亡

　　[Abstract] Objective To investigate the relationship between EZH2 and age-related islet cell function. Methods Healthy SD rats were divided into 4 groups (n=6 for each group) by age: 1, 6 , 12, 24 months of age groups respectively. Pancreases were taken out for immunohistochemical staining. The protein levels of EZH2 and insulin were determined by immunohistochemistry and Western blot. Islet cells proliferation and apoptosis were detected by Ki67 staining and TUNEL staining respectively. Results With age increasing, the expression of EZH2 in islet was gradually decreased:1 month of age group(0.22516±0.03091);6 months of age group (0.18427±0.02315);12 months of age group(0.03165±0.01675);24 months of age group (0) (P<0.01). Proliferation of islet cells was also gradually decreased: 6 months of age group(0.36±0.03)%;12 months of age group (0.61±0.02)%;24 months of age group (0.12±0.02)%(P<0.01). Apoptosis of islet cells was gradually increased：12 months of age group (0.02±0.03)%;24 months of age group( 0.09±0.04)%(P<0.01). And with the increase of age , the expression of insulin in the iset(islet)：1 month of age group (206.5±27.8);6 months of age group (267.4±25.3);12 months of age group (376.2±31.9);24 months of age group (187.8±25.1)(P<0.01). Conclusion With increasing age, the islet cells were gradually aging, and the expression of EZH2 was gradually reduced in aging islet cells, accompanied by decreased islet cell proliferation, apoptosis, and gradually reduced insulin levels.

　　[ Keywords ] Enhance of Zeste homolog 2;Proliferation of islet cells; Apoptosis