▪糖尿病基础研究▪

　　　　【摘要】 目的 观察二甲双胍对系膜细胞(MCs)核因子-κB(NF-κB)，单核细胞趋化蛋白-1(MCP-1)表达合成的影响，探讨其肾脏保护机制。 方法 体外培养MCs，随机分为正常糖(Con)组、高糖(HG)组及高糖+不同浓度二甲双胍(M1、M2、M3)组。48 h后收集各组细胞及上清液，采用荧光实时定量聚合酶链式反应(RT-PCR)检测细胞NF-κB, MCP-1 mRNA含量，蛋白印迹法(Western blot)检测NF-κBp65蛋白水平，ELISA检测上清MCP-1蛋白浓度。 结果 (1)与Con组比较，HG组NF-κB mRNA和MCP-1 mRNA的表达增加(P<0.05);NF-κBp65的相对表达量增加(1.04±0.01 vs 0.51±0.15, P<0.05)，上清MCP-1水平增高[(561.99±23.24) pg/ml vs (480.73±35.40) pg/ml, P<0.05];(2)二甲双胍干预后，NF-κB mRNA、MCP-1 mRNA表达及NF-κBp65蛋白、上清MCP-1含量均下降(P<0.05)。 结论 二甲双胍可抑制MCs NF-κB和MCP-1的表达合成，可能与其肾脏保护作用有关。

　　   【关键词】 二甲双胍;核因子-κB;单核细胞趋化蛋白-1

　　　【Abstract】 Objective To observe the effects of metformin on expression and synthesis of nuclear factor-κB and monocyte chemoattractant protein-1 in rat glomerular mesangial cells(GMC cell) and explore its reno-protective mechanisms. Methods GMC cell were cultured in the medium with normal glucose(Con group) ,high glucose(HG group) and different concentrations of metformin (M1，M2，M3 group). After 48 h exposure, the supernatants and GMC cell were collected. The mRNA expression of NF-κB and MCP-1 was analyzed by real-time PCR and MCP-1 protein was synthesized by ELISA, while NF-κB p65 was observed by Western blot . Results (1)After stimulation of high glucose,the mRNA levels of NF-κB and MCP-1,and protein content of NF-κBp65 and MCP-1 were significantly increased in other groups as compared with Con group (P<0.05). (2) The levels of intracellular NF-κB mRNA, MCP-1 mRNA, protein content of NF-κBp65 and MCP-1 were significantly decreased in M1，M2，M3 group as compared with group HG. Conlusions Metformin can suppress the expression of NF-κB mRNA and MCP-1 mRNA, and MCP-1 protein synthesis of glomerular mesangial cells, which may partly contribute to its reno-protection.

　　【Key words】 Metformin;NF-κB;MCP-1