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甘氨酸保护果糖饮食诱导大鼠糖尿病机制的研究

来自:中国糖尿病杂志  编辑:任云峰 宋彬妤 姚心琪 吴惠文|点击数:|2014-04-25

  ·糖尿病基础研究·

  【摘要】 目的 探讨果糖饮食致大鼠发生T2DM,肠源性内毒素血症(IETM)发挥的作用及甘氨酸的保护机制。 方法 将32只SD大鼠随机分为对照组(NC,n=8),果糖模型(F,n=8)组,甘氨酸干预(F+G,n=8)组,及甘氨酸(G,n=8)组。大鼠自由饮水、进食(标准大鼠饲料)F组给予8%果糖水喂养,F+G组给予8%果糖+1%甘氨酸水喂养。4月末行OGTT后处死,检测内毒素(LPS)、FPG、TNF-α、IL-6及FIns;计算胰岛素抵抗指数(HOMA-IR)及β细胞功能指数(HOMA-β);Western blot检测脂肪组织NF-κB表达。 结果 F组出现IETM、IR、IGT及胰岛β细胞功能障碍,FPG、TNF-α、IL-6、FIns、NF-κB表达均高于NC组(P < 0.01),F+G组上述指标均有改善(P<0.05)。血浆LPS与HOMA-IR呈正相关(rs=0.76,P=0.0164)。 结论 IETM通过炎症机制参与T2DM发病,甘氨酸减轻IETM,对T2DM有一定的保护作用。

  【关键词】 果糖饮食;糖尿病,2型;肠源性内毒素血症;甘氨酸;胰岛素抵抗

Glycine protect against type 2 diabetes in rats induced by high fructose diet  REN Yun-feng, SUNG Bing-yu, YAO Xin-qi, et al. Maternal and Child Care Service Centre of Fenyang, Fenyang 032200, China

Corresponding author: WU Hui-wen, E-mail: wuhuiwen@yeah.net

  【Abstract】 Objective To investigate the roles of intestinal endotoxemia (IETM) in type 2 diabetes (T2DM) rats induced by high fructose-fed diet, and the protective effect of glycine on it. Methods Thirty-two SD rats were randomly divided into normal control(NC,n=8) group, frutose model (F,n=8) group, glycine intervene (F+G,n=8)group and glycine (G,n=8) group. F group was received 8% fructose, and F+G group was received 8% fructose and 1% glycine respectively. The rate were sacrificed at month 4. Endotoxin (LPS), fasting blood glucose (FBG), fasting insulin (FIns) and pro-inflammatory cytokines were measured. HOMA-IR and HOMA-β were calculated. Western blot was used to determine the expression of NF-κB in adipose tissue. Results The levels of LPS, FBG, FIns, pro-inflammatory and HOMA-IR increased (P<0.01), HOMA-β was reduced (P<0.01), and glucose tolerance was impaired, the expression of NF-κB was significantly up regulated in the F group as compared with the NC group. But the indexes above mentioned in F+G group were partially ameliorated. Conclusion Intestinal endotoxemia contributes to T2DM by inflammatory mechanisms, and glycine has significantly protective effects via alleviating intestinal endotoxemia in T2DM rats.

  【Key words】 High- fructose diet; Diabetes, type 2; Intestinal endotoxemia; Glycine; Insulin resistance

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