糖尿病神经病变·

　　【摘要】 目的 探讨糖尿病周围神经病变(DPN)患者血清脑源性神经营养因子(BDNF)与高迁移率族蛋白B1(HMGB1)水平的相关性。 方法 将T2DM患者60例分为单纯T2DM组和合并DPN组，各30例，另选取健康体检者30名作为对照(NC)组。ELISA检测血清BDNF及HMGB1含量，实时定量PCR法检测其mRNA在外周血单个核细胞中的相对表达水平，分析DPN患者中BDNF与HMGB1相关性。 结果 T2DM组和DPN组血清BDNF含量及mRNA表达水平均低于NC组(P<0.05)，而HMGB1含量及mRNA表达水平高于NC组(P<0.05)。DPN组患者血清BDNF含量及mRNA表达水平低于T2DM组，血清HMGB1含量及mRNA表达水平高于T2DM组(P<0.05)。DPN组血清BDNF与HMGB1相关(r=-0.425,P<0.05)。 结论 DPN患者血清BDNF与HMGB1相关，二者可能共同参与了DPN的发病。

　　【关键词】 糖尿病，2型;糖尿病周围神经病变;高迁移率族蛋白B1;脑源性神经营养因子

　　【Abstract】 Objective To investigate the association between serum brain-derived neurotrophic factor (BDNF) and high-mobility group box-1 (HMGB1) levels in T2DM patients with peripheral neuropathy. Methods Sixtiy patients with type 2 diabetes mellitus were divided into T2DM group (n=30) and DPN group (n=30). 30 healthy subjects were collected as normal control (NC) group. The serum levels of BDNF and HMGB1 were tested by ELISAs, BDNF and HMGB1 mRNA were detected by quantitative real-time RT-PCR in peripheral blood mononuclear cells. The correlation analysis was carried out between BDNF and HMGB1. Results The serum level of BDNF and the expression level of mRNA were lower in T2DM and DPN groups than in NC group (P<0.05), whereas the serum level of HMGB1 and its mRNA expression was higher. The serum level of BDNF and its mRNA expression level of mRNA were lower, but the serum level of HMGB1 and expression level of mRNA was higher in DPN group than in T2DM group(P<0.05). In DPN group, correlation analysis showed a significant relationship between BDNF and HMGB1(r=-0.425,P<0.05). Conclusion There is a significant correlation between the levels of HMGB1 and BDNF in type 2 diabetic patients with peripheral neuropathy. Both BDNF and HMGB1 may participate in the process of type 2 diabetic patients with peripheral neuropathy.

　　【Key words】 Diabetes mellitus，type 2;Diabetic peripheral neuropathy;High-mobility group box-1;Brain-derived neurotrophic factor