·糖尿病基础研究·

　　【摘要】 目的 观察螺内酯对T2DM大鼠肾脏组织紧张素转换酶2(ACE2)和细胞间黏附分子(ICAM-1)表达的影响，以探讨其对糖尿病肾脏病变的保护作用。方法 用高脂高糖饲料喂养大鼠2个月后，腹腔注射 30 mg/ kg体重的链脲佐菌素制备糖尿病大鼠模型 ,分为正常对照组 (NC组)、糖尿病组 (DM组)、螺内酯治疗组 (R组),检测第16周FBG、Cr、24 hUAER、肾重/体重(×10-3);并用免疫组化和RT-PCR的方法检测16周后大鼠肾脏ACE2和 ICAM- 1表达的改变。结果 第16周末FBG DM组和 R组相近，差异无统计学意义(P>0.05) ,但DM组和 R组均显著高于NC组( P < 0.01) ; ACE2水平R组显著高于DM组( P < 0.01)，I CAM- 1水平 R组低于 DM组 (P < 0.05) 。结论 螺内酯能通过增加肾脏组织ACE2的表达、降低肾脏组织ICAM- 1的表达而发挥保护肾脏的作用。

　　【关键词】 糖尿病大鼠;糖尿病肾病;血管紧张素转换酶2 ;细胞间黏附分子1

Effect of spironolactone on the expressions of ACE2 and ICAM-1 in the kidney of T2DM rats ZHOU Xiang-ran, HAN Gang, SUN Kan, et al. Medical School of Shihezi University, Shihezi 832000, China

Corresponding author: HAN Gang, E-mail: hangws@163.com

　　【Abstract】 Objective To observe the effect of spironolactone on the expressions of angiotensin-converting enzyme 2 (ACE2) and intercellular adhesion molecular -1 (ICAM-1) in the kidney of T2DM rats and to discuss its protection against diabetic nephropathy. Methods The rat models of T2DM were produced by peritoneal injection of streptozotocin (STZ) 30mg/kg after feeding high-sugar and high-fat diet for two months. The rats were assigned to normal control (NC) group, diabetic rat (DM) group, and spironolactone treatment (R) group. At the end of 16th week, blood glucose (BG), Creatinine (Cr), 24-hour urine albumin excretion rate (U-AER), and kidney weight/body weight (×10-3) were detected. The expressions of ACE2 and ICAM-1 were measured with immunohistochemical method after 16 weeks. Results At the end of the 16th week, the fasting blood glucose level (FBG) in the DM group was similar to that of the R group (P>0.05), but it was significantly higher in the DM group and NC group than in the NC group (P<0.01); the expression of ACE2 in the R group was significantly higher than in the DM group (P<0.01), while the expression of ICAM-1 in the R group was significantly lower than in the DM group (P<0.05). Conclusion Spironolactone plays a protective role on the kidneys by up-regulating ACE2 and down-regulating ICAM-1.

　　【Key Words】 Diabetic rat; Diabetic nephropathy; Angiotensin-converting enzyme 2 (ACE2); Intercellular adhesion molecular -1 (ICAM-1)