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罗格列酮对2型糖尿病大鼠肾脏ERK/c-fos蛋白表达的影响

来自:中国糖尿病资讯网  编辑:admin|点击数:|2011-08-25

  ·糖尿病基础研究·

  王隽 王战建 徐焕宇

  作者单位:101149首都医科大学附属北京胸科医院(王隽);河北医科大学第三医院内分泌科(王战建、徐焕宇)

  通讯作者:王战建,E-mail: wishsstars@163.com

  【摘要】 目的 观察罗格列酮对糖尿病大鼠肾脏细胞外信号调节激酶(ERK)、c-fos蛋白表达的影响。 方法 应用高糖高脂饮食加小剂量链脲佐菌素建立2型糖尿病大鼠模型,用免疫组化 方法 检测肾脏ERK、c-fos蛋白表达。 结果 (1)成模动物具有高血糖、胰岛素抵抗、肾脏病变等特点。(2)糖尿病组大鼠肾组织中ERK、c-fos表达较对照组明显增加。罗格列酮治疗后ERK、c-fos活性降低,肾功能及组织病理学损害改善。 结论 2型糖尿病大鼠模型肾组织ERK/c-fos蛋白表达增加。罗格列酮治疗后ERK/c-fos表达降低,伴肾功能和肾脏病理损害改善。

  【关键词】罗格列酮;动物模型;细胞外信号调节激酶;c-fos

  doi:10.3969/j.issn.1006-6187.2011.08.019

  Effect of rosiglitazone on protein expression of renal ERK and c-fos in the type 2 diabetic rats

  WANG Jun, WANG Zhan-jian, XU Huan-yu. Beijing Chest Hospital Affiliated to Capital Medical University, Beijing 101149, China

  Corresponding author: WANG Zhan-jian,E-mail: wishsstars@163.com

  【Abstract】 ObjectiveTo investigate the effect of rosiglitazone on activation of renal ERK and c-fos in the experimental type 2 diabetic rats. Methods The rats of model groups were intraperitoneally given low-dose streptozotocin(STZ) after taking the sucrose-and fat-rich diets for one month. Immunohistochemistry and computer image-pattern analysis system were used to analyze expression of ERK and c-fos in renal tissues. Results (1)This rat model of type 2 diabetes mellitus showed hyperglycemia, IR and typical renal lesions. (2)Compared to normal group, the protein expression of ERK and c-fos were increased in glomeruli of type 2 diabetic rats. Rosiglitazone inhibited significantly the activation of ERK and c-fos, partly improved the renal function and matrix accumulation. Conclusions (1) A rat model with type 2 diabetes mellitus presents the increased protein expression of ERK and c-fos of diabetic nephropathy. (2) The kidney protection of rosiglitazone is associated, at least in part, with down-regulating ERK/c-fos activation in renal tissues.

  【Key words】Rosiglitazone; Animal model; ERK; c-fos

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