·糖尿病基础研究·

李如江 赵春艳 高海玲 管英俊

基金项目：山东省自然科学基金资助项目(Y97C05041);山东省医药卫生科研资助项目(HW153)

作者单位：261053山东，潍坊医学院

通讯作者：管英俊，E-mail： lingshangfei@163.com

【摘要】 目的 研究1型糖尿病(T1DM)小鼠胰岛β细胞量的变化及变化机制。 方法 糖尿病第4、12、20周时，T1DM小鼠被放血处死。以Insulin、Krüppel-like factor 4(KLF4)、Caspase-3、TUNEL的免疫荧光标记及再生蛋白1 mRNA半定量RT-PCR检测β细胞量、β细胞再生和凋亡。结果 β细胞量随糖尿病发病时间延长逐渐减少，至糖尿病第12周时降至最低，第20周与第12周比较无统计学差异。β细胞量的变化与其Caspase-3、KLF4的表达和胰腺再生蛋白1 mRNA的丰度密切相关。 结论 1型糖尿病鼠胰岛β细胞并未完全丧失，其机制与β细胞凋亡减少、再生增加密切相关。

【关键词】 糖尿病,1型,β细胞量,凋亡,小鼠

doi:10.3969/j.issn.1006-6187.2011.07.019

The change of β cell mass and its mechanism in type 1 diabetic mice

LI Ru-jiang, ZHAO Chun-yan, GAO Hai-ling, et al. Weifang Medical University, Weifang 261053, China

Corresponding author: GUAN Ying-jun, E-mail: lingshangfei@163.com

【Abstract】 Objective To explore the change of β cell mass and its mechanism in type 1 diabetic mice. Method sType 1 diabetic mice were killed by exsanguination after 4, 12 and 20 weeks of diabetes, respectively. Indirect immunofluorescences for insulin, Krüppel-like factor 4 (KLF4), caspase-3 and TUNEL and semi-quantitative RT-PCR for pancreatic regenerating protein 1 mRNA were used to evaluate pancreatic β cell mass, β cell regeneration and apoptosis. Result sThe β cell mass was gradually decreased in diabetic mice along with time until 12-week of diabetes. No significant difference in β cell mass between 12-week and 20-week of diabetic course was demonstrated. Such change of β cell mass was associated with the variances of caspase-3, KLF4 in β cells and the regenerating protein 1 mRNA expression in pancreata. Conclusion The β cell mass is not totally lost in type 1 diabetic mice which seems to be strongly associated with attenuated β cell apoptosis and with improved β cell neogenesis.

【Key words】 Diabetes mellitus, type 1; β cell mass; Apoptosis; Mouse