·糖尿病基础研究·

李鹏杰 李强 潘红艳 郭琳 于萍 张巾超

基金项目：教育部高等学校博士学科点科研基金(200802260007);黑龙江省教育厅高等学校骨干教师创新计划(1054G024)

作者单位：150086哈尔滨，哈尔滨医科大学附属第二医院内分泌代谢病科

通讯作者：李强，E-mail:hrblq@yahoo.com.cn

【摘要】 目的 研究不同浓度葡萄糖和胰岛素对3T3-L1脂肪细胞中内脏脂肪素(Visfatin)mRNA表达的影响。 方法 通过real-time RT-PCR 方法检测不同浓度葡萄糖和胰岛素培养下3T3-L1脂肪细胞Visfatin mRNA的表达。 结果 葡萄糖增加了3T3-L1脂肪细胞Visfatin mRNA的表达;胰岛素降低其表达。 结论 葡萄糖和胰岛素对3T3-L1脂肪细胞中Visfatin mRNA的表达有调控作用。

【关键词】 内脏脂肪素,葡萄糖,胰岛素,3T3-L1脂肪细胞

doi:10.3969/j.issn.1006-6187.2011.07.018

Effects of glucose and insulin on the expressions of visfatin mRNA in 3T3-L1 adipocytes

LI Peng-jie,LI Qiang,PAN Hong-yan,et al.Department of Endocrinology,the 2nd Affiliated Hospital of Harbin Medical Univercity,Harbin 150086,China

Corresponding author:LI Qiang,E-mail:hrblq@yahoo.com.cn

【Abstract】 Objective To investigate the effects of glucose and insulin in different concentrations on the expressions of visfatin mRNA in 3T3-L1 adipocytes. Method s3T3-L1 adipocytes were treated with different concentration of glucose and insulin.The expressions of visfatin mRNA were measured by quantitative real-time RT-PCR with 36B4 as internal control. Result sGlucose increased the expression of visfatin mRNA,but insulin downregulated visfatin mRNA expressions in 3T3-L1 adipocytes. Conclusion sGlucose and insulin regulate the expression of visfatin mRNA in 3T3-L1 adipocytes.

【Key words】 Visfatin;Glucose;Insulin;3T3-L1 adipocytes