来自:中国糖尿病资讯网 编辑:lj|点击数:|2011-10-20
血红素加氧酶1在高糖和糖基化终产物致人单核细胞氧化应激中的作用
关美萍 薛耀明 卓凤婷 沙建平 高方 何飞英 王玲
·糖尿病基础研究·
作者单位:510515广州,南方医科大学南方医院内分泌代谢科
通讯作者:薛耀明,E-mail: bright.x@medmail.com.cn
【摘要】 目的
探讨血红素加氧酶1(HO-1)高表达在对抗高糖和糖基化终产物(AGEs)诱导的人单核细胞(THP-1)氧化应激中的作用。 方法 THP-1细胞分为对照(NC)组、GLU+AGEs组、钴原卟啉(CoPP)+GLU+AGEs组和CoPP组4组,孵育24 h后收集细胞及培养液上清,检测各组细胞的活性氧(ROS)产量、培养液上清丙二醛(MDA)和肿瘤坏死因子α(TNF-α)水平及HO-1表达。 结果 GLU+AGEs组的ROS产量、MDA和TNF-α水平均显著高于NC组(P<0.05);CoPP+GLU+AGEs组的ROS产量和MDA水平均显著低于GLU+AGEs组(P<0.05)。GLU+AGEs组的HO-1基因和蛋白表达均显著高于NC组(P<0.01),CoPP+GLU+AGEs组的HO-1蛋白表达显著高于GLU+AGEs组(P<0.01)。 结论 CoPP通过诱导HO-1蛋白高表达拮抗高糖和AGEs导致的THP-1细胞氧化应激,通过诱导HO-1高表达可能拮抗糖尿病所致单核细胞氧化应激。
doi:10.3969/j.issn.1006-6187.2011.06.018
Effects of CoPP on oxidative injury induced by high glucose and AGEs in human monocyte THP-1 cells
GUAN Mei-ping, XUE Yao-ming, ZHUO Feng-ting, et al. Department of Endocrinology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Corresponding author:XUE Yao-ming,E-mail: bright.x@medmail.com.cn
【Abstract】 Objective
To investigate the effects of CoPP(cobalt protoporphyrin, being an inducer of heme oxygenase-1, HO-1 as an antioxygen agent) on oxidative injury in human monocyte THP-1 cells incubated with high glucose and advanced glycation end products (AGEs). Methods There were four groups: control group, GLU+AGEs group (15 mmol/L glucose+100 μg/mL AGEs), CoPP group (5.5 mmol/L glucose+10 μmol/LCoPP) , CoPP+GLU+AGEs group (10 μmol/L CoPP+15 mmol/L glucose+100 μg/mL AGEs). Reactive oxygen species (ROS), malondialdehyde (MDA) and tumor necrosis factor α (TNF-α) were determined and the expressions of HO-1 mRNA and protein were detected by RT-PCR and Western blotting, respectively. Results ROS output and MDA were significantly lower in CoPP+GLU+AGEs gorup (95.42±2.36, 1.28±0.61 respectively) than in GLU+AGEs group (107.21±8.9, 3.26±0.32) (all P<0.05). CoPP+GLU+AGEs group had significantly higher expressions of HO-1 protein than did GLU+AGEs group (P<0.05). Conclusion s The oxidative injury and inflammation in human monocytoid THP-1 cells induced by high glucose and AGEs are suppressed by HO-1 inducer.The agents inducing high expression of HO-1 may antagonize the diabetes-induced oxidative injury.
【Key words】Heme oxygenase-1; Cobalt protoporphyrin; Advanced glycation end products; Oxidative stress; Huamn monocyte THP-1 cell line
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