不同浓度葡萄糖和胰岛素对结直肠癌细胞株Colon26肿瘤细胞分泌白介素6影响的体外研究

杨爱格 周慧敏 张庆富 王润田

·糖尿病基础研究·

基金项目：河北省重大疾病专项(07276103D)

作者单位：050031石家庄，河北医科大学第一医院内分泌科

通讯作者：周慧敏，E-mail:zhouhuimindoctor@163.com

【摘要】 目的 观察不同浓度的葡萄糖和胰岛素对结直肠癌细胞株Colon26肿瘤细胞分泌白介素6(IL-6)的影响。 方法 体外常规培养Colon26肿瘤细胞。根据所用培养液外加葡萄糖和胰岛素终浓度的不同，将实验所用细胞分为4组(12亚组)，培养48 h，收集细胞培养上清，ELISA法检测IL-6浓度。 结果 葡萄糖浓度为25mmol/L的亚组和其加入不同浓度的胰岛素的亚组细胞培养上清中IL-6的浓度明显高于对照组(P<0.01);其他亚组与对照组相比均无显著差异(P>0.05)。 结论 25mmol/L的葡萄糖可显著增加Colon26肿瘤细胞分泌IL-6;不同浓度的胰岛素对Colon26肿瘤细胞分泌IL-6无明显影响。

doi:10.3969/j.issn.1006-6187.2011.06.016

The effect of different concentrations of glucose and insulin on secretion of IL-6 in colon26 cells in vitro

YANG Ai-ge,ZHOU Hui-min, ZHANG Qing-fu, et al. Department of Endocrinology，The First Hospital of Hebei Medical University, Shijiazhuang 050031, China

Corresponding author: ZHOU Hui-min,E-mail: zhouhuimindoctor@163.com

【Abstract】 Objective To observe the effects of different concentrations of glucose and insulin on the secretion of IL-6 in colon26 tumor cells. Methods Colon26 tumor cells were cultured in vitro. The different concentrations of glucose and insulin were added into culture medium and the cells were divided into 4 groups(12 subgroups). After 48h of incubation,

the superantants were collected. In all experiments IL-6 concentration in the supernatant was detected by ELISA. Results

The level of IL-6 were significantly higher in 25 mmol/L glucose than in control group(P<0.01). There was no statistical difference in the secretion of IL -6 in colon26 tumor cells between groups of control and glucose/insulin subgroups except for 25 mmol/L glucose (P>0.05). Conclusion s High concentration of glucose may up-regulate the secretion of IL-6 in Colon26 tumor cells, while different concentrations of insulin have no effect on the secretion of IL-6 in these cells.

【Key words】Glucose;Insulin;IL -6;Diabetes mellitus;Colorectal cancer