白介素18基因型及血清水平与2型糖尿病肾病的相关性

　　陈军宁 尹友生 李小励 韦家智 陈媛媛 李康惠 苏珂

　　·糖尿病性肾脏疾病·

　　基金项目：广西壮族自治区卫生厅课题 (Z2005163 )

　　作者单位： 541000桂林,桂林医学院附属医院泌尿内科(陈军宁、尹友生、李小励、韦家智、陈媛媛、李康惠)，内分泌科(苏珂)

　　【摘要】 目的 研究白介素18(IL-18) 基因型及血清水平与2型糖尿病肾病(DN)的关系。 方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术，检测360例2型糖尿病患者(160例伴DN) 及180名正常对照组的IL-18的基因多态性，同时采用酶联免疫吸附试验(ELISA)检测血清IL-18水平。 结果 与对照组比较，糖尿病肾病组血清IL-18水平显著高于对照组(P<0.01)。 IL-18基因-137 G/C多态性在两组人群中的分布差异有统计学意义(P<0.05)，携带-137 C等位基因的糖尿病肾病患者血清IL-18水平显著高于不携带者(P<0.05)。 结论 IL-18基因-137 G/C多态性与DN的发病可能具有相关性，其中C等位基因可能是DN发病的遗传易感基因;携带C等位基因的个体可能通过促进IL-18的高度表达进而增加了DN的发病风险。

　　doi:10.3969/j.issn.1006-6187.2011.06.005

　　The relationship of serum level and the genotype of interleukin-18 with diabetic nephropathy in type 2 diabetes mellitus

　　CHEN Jun-ning, YIN You-sheng, LI Xiao-li, et al. Department of Emergency, The Affiliated Hospital of Guilin Medical college，Guangxi Guilin，541001 China

　　【Abstract】 Objective To study the relationship of the serum levels and genotype of IL-18 with diabetic nephropathy in T2DM. Methods The polymorphisms of IL-18 gene were analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 360 patients with type 2 diabetes mellitus and 180 healthy controls. And the serum level of IL-18 was determined by enzyme-linked immunosorbent assay(ELISA). Results The diabetic nephropathy group showed significantly higher serum levels of IL-18 than control group (P<0.01). The IL-18 gene -137G/C polymorphism was significantly different between type 2 diabetic nephropathy and control group. The serum level of -137 C allele carriers was significantly higher than non-carriers (P<0.05). Conclusion IL-18 gene -137G/C polymorphism is associated with diabetic nephropathy in T2DM, and C allele may be a risk factor for diabetic nephropathy. The IL-18 C allele carriers may increase the risk for pathogenesis of type 2 diabetic nephropathy by enhancing the IL-18 expression.

　　【Key words】Diabetic nephropathies; interleukin-18; Genotype