基金项目：国家自然科学基金项目(20805026，90709045);国家973计划课题(2005CB523503);科技部国际合作项目(2010DFA32420);国家“十一五”科技支撑计划(2006BA104A04-02)

作者单位：330004南昌，江西中医学院(于欢、龚千锋);清华大学分析中心(梁琼麟、王义明、罗国安);广州中医药大学第二临床医学院中心实验室，博士后科研流动站(黎莉);中国中医科学院广安门医院(仝小林);北京协和医院(肖新华);鹤岗市人民医院(屈克义);宜昌市夷陵医院(刘杰)

通讯作者：罗国安

天芪降糖胶囊对糖耐量减低患者

脂肪代谢组学的影响

于欢梁琼麟黎莉王义明龚千锋仝小林罗国安

肖新华屈克义刘杰

【摘要】目的研究天芪降糖胶囊对糖耐量减低患者血清代谢物的影响，探讨天芪降糖胶囊的作用途径。方法应用UPLC/TOF-MS液质联用分析方法，建立人血清代谢指纹谱，进行主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)，观察天芪降糖胶囊的干预效果。结果治疗3个月、6个月后受试者血清代谢物发生了明显变化，天芪降糖胶囊参与机体在磷脂、糖脂、核苷、肉碱等代谢水平的修复过程。结论应用代谢组学方法，可以从整体出发研究天芪降糖胶囊对机体的作用，从小分子代谢物变化衡量其疗效。

【关键词】天芪降糖胶囊;糖耐量减低;代谢组学;超高效液相色谱-飞行时间质谱

doi:10.3969/j.issn.1006-6187.2011.05.008

Effects of Tianqijiangtang capsule on lipid metabolomics in impaired glucose tolerance (IGT) volunteers

YU Huan, LIANG Qiong-lin, LI Li，et al. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China

Corresponding author:LUO Guo-an

【Abstract】ObjectiveTo study the effects of tiangijiangtang capsule on lipid metabolomics in impaired glucose tolerance patients. MethodsA UPLC/TOF-MS based methods were used for serum metabolic fingerprinting analysis. Multivariate statistical analysis was used to analyze the metabonome difference.

ResultsThe statistically significant changes were found in levels of lipids, acylcarnitine, glycosphingolipid and glucuronidation (all P<0.05) after the treatment with tianqijiangtang capules for 3 and 6 months as compared with untreated people. ConclusionThe UPLC/TOF-MS based metabonomic approach can give some explain to the pathophysiology of impaired glucose tolerance, as a supplement for clinical diagnosis and therapy.

【Key words】Tianqijiangtang capsule; Impaired glucose tolerance; Metabonomics; UPLC/TOF-MS