·糖尿病分子生物学与遗传学·

　　石建霞 邹大进

　　作者单位：200433上海，第二军医大学长海医院内分泌科

　　通讯作者：邹大进，E-mail: zwjd22@medmail.com.cn

　　【摘要】 目的 探讨新诊断的2型糖尿病(T2DM)患者脂肪组织SIRT1 mRNA表达水平及其与体质指数(BMI)、腰臀比(WHR)、血糖、血浆胰岛素、胰岛素抵抗指数(HOMA-IR)关系。 方法 采用RT-PCR方法检测了40例对照组和40例T2DM患者脂肪组织SIRT1 mRNA水平，并分析了SIRT1水平与BMI、WHR、血脂、HbA1c、血糖、血浆胰岛素和HOMA-IR等的关系。 结果 新诊断的T2DM患者SIRT1 mRNA水平显著低于对照组(1.49±0.47 vs 1.12±0.32，P<0.01);线性相关分析表明，SIRT1与FIns、HOMA-IR呈显著负相关(r=-0.421，P<0.01和r=-0.511，P<0.01)。以SIRT1为因变量，年龄、WHR、BMI、TG、TC、LDL-C、HDL-C、HbA1c、FPG、FIns和HOMA-IR为自变量，进行多元线性逐步回归分析，结果表明HOMA-IR是影响SIRT1的独立相关因素。Logistic回归分析表明控制性别、年龄、WHR、BMI、TC、TG、HDL-C、LDL-C后，发现SIRT1与T2DM发病呈负相关，OR<1。 结论 脂肪组织中SIRT1 mRNA水平的变化与IR和T2DM相关。

　　【关键词】糖尿病,2型;胰岛素抵抗;SIRT1

　　doi:10.3969/j.issn.1006-6187.2011.03.005

　　The expression and its significance of SIRT1 mRNA in adipose tissue in patients with newly diagnosed type 2 diabetes mellitus

　　SHI Jian-xia, ZOU Da-jin. Department of Endocrinology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China

　　Corresponding author： ZOU Da-jin, E-mail: zwjd22@medmail.com.cn

　　【Abstract】 Objective To investigate the expression of SIRT1 and the relationship of SIRT1 levels with body mass index(BMI), waist hip ratio(WHR), blood glucose, plasma insulin levels as well as other factors in newly diagnosed type 2 Diabetic mellitus(T2DM). Methods IRT1m RNA in adipose tissue were detected by RT-PCR. The relationship of SIRT1 levels with BMI, WHR, HOMA-IR, blood lipids, plasma glucose, insulin, HbA1c levels were analyzed. Results IRT1 level was higher in T2DM group than in controls(1.49±0.47 vs 1.12±0.32, P<0.01). SIRT1 level was negatively correlated with FIns and HOMA-IR(r=-0.421, P<0.01; r=-0.511，P<0.01). Multiple regression analysis showed that HOMA-IR was an independently related factor influencing SIRT1 level. Binomial Logistic regression analysis indicated that after controlling sex, age, WHR, BMI, TG, TC, LDL-C, HDL-C, the SIRT1 level was negatively correlated with T2DM. Conclusion The SIRT1 expression level is associated with insulin resistance and T2DM.

　　【Key words】Diabetes mellitus, type 2; Insulin resistance; SIRT1