王攀攀 张荣华 朱晓峰 杨丽

　　作者单位：510632广州，暨南大学医学院

　　【摘要】目的 探讨高糖对体外培养小鼠前体成骨细胞MC3T3-E1活性的影响。 方法 将培养的MC3T3-E1细胞分为5.5 mmol/L正常糖对照组、12.5 mmol/L高糖组和25 mmol/L高糖组，观察不同浓度糖对MC3T3-E1细胞增殖、碱性磷酸酶(ALP)、矿化结节、骨钙素(OC)及Caspase-3的影响。 结果 与5.5 mmol/L正常糖对照组相比，12.5 mmol/L高糖组培养5 d细胞增殖和ALP分泌不受影响，在培养7 d和14 d时明显促进矿化结节形成，在培养5 d时促进细胞内OC表达，在培养3 d时促进Caspase-3的表达;而25 mmol/L高糖组在培养5 d则可明显抑制细胞增殖及ALP分泌，在培养14 d减少矿化结节的形成，在培养第5 d时减少OC表达，在培养3 d时促进Caspase-3的表达。 结论 高糖可影响MC3T3-E1细胞活性，12.5 mmol/L糖在培养时间内不影响细胞增殖与分化，可促进矿化;25 mmol/L糖则对细胞产生明显的抑制效应。

　　【关键词】高糖;成骨细胞;MC3T3-E1

　　doi:10.3969/j.issn.1006-6187.2011.02.019

　　Study on effects of high glucose on the activity of osteoblastic cells (MC3T3-E1)

　　WANG Pan-pan, ZHANG Rong-hua, ZHU Xiao-feng, et al. Medical College of Ji′nan University, Guangzhou 510632, China

　　【Abstract】 Objective To investigate the effect of high glucose on the activity of MC3T3-E1 cells. Methods MC3T3-E1 cells were divided into three groups: 5.5 mmol/L normal glucose control group, 12.5 mmol/L high glucose group and 25 mmol/L high glucose group. The effects of different concentrations of glucose on the MC3T3-E1 cells proliferation, ALP, mineralized nodules, osteoclastic cell (OC), and caspase-3 were measured.ResultsCompared with 5.5 mmol/L normal glucose control group, 12.5 mmol/L group had no effect on cell proliferation and ALP secretion (5d), but it can promote cell mineralization(7d, 14d). The secretion of OC (osteocalcin) was increased. However, the expression of caspase-3 was also increased. 25 mmol/L glucose group had a negative impact after 5 days treatment and the secretion of caspase-3 was also promoted. Conclusion High glucose can affect the activity of MC3T3-E1 cell. 12.5 mmol/L glucose had no effect on cell differentiation and proliferation while it can promote cell mineralization. However 25 mmol/L glucose may have an inhibition effect during long-term treatment.

　　【Key words】High glucose; Osteoblast; MC3T3-E1