魏琼 孙子林 董莉 刘必成 阮雄中 刘乃丰

　　作者单位：210009南京，东南大学附属中大医院内分泌科(魏琼、孙子林、董莉)，肾脏病研究所(刘必成)，心血管病研究所(刘乃丰);Renal Unit, Royal Free Hospital, LONDON, UK(阮雄中)

　　通讯作者：孙子林，E-mail: sunzilin@hotmail.com

　　【摘要】目的 研究血管紧张素受体Ⅱ(ATⅡ)的AT1受体阻滞剂伊贝沙坦对2型糖尿病(T2DM)大鼠肾皮质趋化因子fractalkine表达的影响。 方法 制备T2DM大鼠模型，随机分为正常对照组、糖尿病组及伊贝沙坦治疗组。分别用半定量RT-PCR和免疫组化法检测肾皮质fractalkine mRNA和蛋白表达。 结果 糖尿病组血清糖基化终产物-肽、尿素氮(BUN)、24h尿总蛋白(24hUPro)、肾皮质fractalkine mRNA和蛋白表达均明显高于对照组;伊贝沙坦治疗组血清BUN、24hUPro、肾皮质fractalkine mRNA和蛋白表达则明显低于糖尿病组。 结论 伊贝沙坦可能通过抑制肾皮质fractalkine表达发挥其肾保护作用。

　　【关键词】　糖尿病肾病;Fractalkine;伊贝沙坦;糖基化终产物-肽

　　doi:10.3969/j.issn.1006-6187.2011.02.016

　　Effect of Irbesartan on the expression of fractalkine in renal cortex of type 2 diabetic rats.

　　WEI Qiong，SUN Zi-lin，DONG Li，et al. Department of Endocrinology， Zhongda Hospital， Southeast University， Nanjing 210009，China

　　Corresponding author:SUN Zi-lin,E-mail:sunzilin@ hotmail.com

　　【Abstract】ObjectiveTo investigate the effect of irbesartan on the expression of fractalkine in renal cortex of type 2 diabetic rats.MethodsType 2 diabetic rats were induced by an intraperitoneal injection of low doses of streptozotocin combined with high-fat diet， and were randomized to receive irbesartan or vehicle treatment.The mRNA and protein levels of fractalkine were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining， respectively.ResultsAGE-P, serum BUN, 24hUPro，the levels of fractalkine mRNA and protein in renal cortex were significantly higher in diabetic rats than in normal control. However, those of rats treated with irbesartan were obviously lower as compared to untreated group.ConclusionsIrbesartan may play the renoprotective role via inhibition of fractalkine expression in the renal cortex.

　　【Key words】

　　Diabetic nephropathy; Fractalkine; Irbesartan; AGE-peptides