杨开明 胡晓松 羊惠君 李爱冬 李茂新

　　基金项目： 大理学院科研启动基金(KY421540)

　　作者单位：671000云南大理，大理学院基础医学院组织学与胚胎学教研室(杨开明、李茂新);成都医学院(胡晓松、羊惠君、李爱冬)

　　【摘要】 目的 研究胰岛β细胞损伤后胰岛再生过程中Wnt/β-catenin信号通路的生物调控。方法 STZ破坏新生大鼠胰岛β细胞，免疫组织化学观察1 d、2 d、3 d 中Wnt/β-catenin通路β-catenin为核心的上、下游基因APC、c-Myc以及Nestin在胰岛再生过程中的表达。 结果 β-catenin在残留的胰岛细胞中表达上调，并在胰腺间质中异常表达;APC表达于胰腺导管上皮及残留的胰岛细胞;c-Myc则主要表达于胰腺腺泡上皮及胰腺间质内;Nestin表达分布于残留的胰岛细胞和胰腺导管上皮。 结论 Wnt/β-catenin信号通路在胰岛再生中可能通过促进胰腺干细胞分化与增殖而调控。

　　【关键词】β细胞;Wnt信号;胰腺干细胞;胰岛再生

　　doi:10.3969/j.issn.1006-6187.2011.02.018

　　The change of Wnt/β-catenin pathway in diabetic rat pancrease and its role in regulating the differentiation and proliferation of pancreatic stem cells

　　YANG Kai-ming, HU Xiao-song, YANG Hui-jun, et al.The Histology & Embryology Department of Basic Medicine School of Dali University，Dali 671000, Chnia

　　【Abstract】 Objective To investigate the regulation role of Wnt/β-catenin signal pathway in regulating pancreatic islets regeneration after β-cells injury.MethodSTZ were used to injure β-cells and imunohistochemistry method was adopted to check the expression of several vital factors e.g. APC, β-catenin, c-Myc for Wnt/β-catenin signal pathway and of nestin in pancreatic islets regeneration. Results Immunohistochemistry showed that the β-catenin was up-regulated in β-cells and abnormally expressed in pancreatic stroma; APC was expressed on duct epithelium and β cells; C-myc was expressed in stroma and gland alveolus epithelium; nestin was expressed on islet cell and duct epithelium.ConclusionThe regulation roles of Wnt/β-catenin signal pathway in pancreatic islets regeneration may be through promoting pancreatic stem cells differentiation and proliferation.

　　【Key words】β-cells ; Wnt signal pathway; Pancreatic stem cell; Pancreatic islets regeneration