·糖尿病基础研究·

　　韩 炜、刘 静、康姚洁

　　作者单位： 730030 兰州市，西北民族大学(韩炜);甘肃省人民医院内分泌科(刘静);第四军医大学(康姚洁)

　　　　【摘要】目的研究不同剂型表没食子儿茶素没食子酸酯(EGCG)对高糖诱导人脐静脉内皮细胞(HUVEC)氧化应激损伤所引起凋亡的抑制作用及人核因子-кB P65(NF-кB P65)表达的影响。方法从新鲜脐带中分离培养HUVEC。用高糖诱导HUVEC表达NF-кB P65，实验组加入不同浓度的EGCG(12.5、 25、 50、 100、 200μmol/L)进行干预，PCR检测NF-кB P65 mRNA的表达，AV-PI 法检测凋亡细胞。结果高糖可诱导HUVEC凋亡,NF-кB P65表达增高;EGCG可抑制NF-κB P65的表达,降低凋亡指数,具有一定的时效关系、剂量关系。结论EGCG可在一定程度上抑制高糖诱导的氧化应激损伤所致的细胞凋亡。EGCG可能是通过抑制高糖所致的NF-κB增高，从而调控HUVEC细胞凋亡过程，发挥对HUVEC的保护作用。

　　【关键词】表没食子儿茶素没食子酸酯;人脐静脉内皮细胞;氧化应激;凋亡;NF-кB P65;高糖

　　doi:10.3969/j.issn.1006-6187.2011.12.019

　　Effect of epigallocatechin-3-gallate on endothelial cells oxidative stress induced by high glucoseHAN Wei, LIU Jing, KANG Yao-jie. Northwest University for Nationalities, Lanzhou 730030, China

　　【Abstract】  Objective   To research the effect of different concentration of epigallocatechin-3-gallate(EGCG) on the endothelial cells oxidative stress induced by high glucose.   Methods   The human umbilical vein endothelial cells (HUVEC) were isolated from umbilical cords.High glucose induced the NF-кB P65 expression on HUVEC cells. Experimental samples were co-cultured with EGCG at different concentrations(12.5,25,50,100,200 μmol/L). The NF-κB P65 mRNA was analyzed by RT-PCR.The apoptoic rate was analyzed by AV-PI stain for flow cytometry(FCM).   Results   High glucose induced the oxidative stress of HUVEC and the apoptosis of HUVEC as compared with normal control group. The up-regulation of NF-кB P65 mRNA expression(P<0.05) occurred from 0.46±0.02 at 12h to 0.72±0.03 at 48h in glucose group(P<0.05). The EGCG(50-200 μmol/L) decreased NF-κB P65 mRNA expression and the apoptotic index in dose-and time-dependent manner. The EGCG can down-regulate the rate of cellular apoptosis.   Conclusions  EGCG decreases apoptosis induced by oxidative stress under high glucose. EGCG protects HUVEC via inhibiting the up-regulation of NF-κB signal transduction pathway induced by high glucose.

　　【Key words】Epigallocatechin-3-gallate;Human umbilical vein endothelial cells;Oxidative stress;Apoptosis;NF-kappa B;High glucose