·糖尿病基础研究·

　　丁和远、刘  军、查  英、盛   励、徐  炯、陈灶萍、查兵兵、王  芳

　　作者单位：200240复旦大学附属上海市第五人民医院内分泌科

　　【摘要】目的  探讨脂联素对高浓度葡萄糖诱导的原代大鼠胰岛β细胞凋亡的影响及其可能机制。方法  分离和培养原代胰岛细胞，分组干预并检测胰岛细胞凋亡率及相关基因表达水平。结果  高糖组胰岛β细胞凋亡率、Caspase-3和Bax mRNA表达量显著高于正常葡萄糖和高糖联合脂联素组(P<0.01)。结论  脂联素可以抑制高糖诱导的大鼠胰岛β细胞凋亡，其作用机制可能与凋亡相关基因Caspase-3与Bax基因表达下调有关。

　　【关键词】脂联素;胰岛细胞;凋亡

　　doi:10.3969/j.issn.1006-6187.2011.12.017

　　Study of adiponectin suppression of high glucose-induced SD rat primary pancreatic β cell apoptosis DING He-yuan, LIU Jun, ZHA Ying, et al. Department of Endocrinology, Shanghai Fifth People′s Hospital, Fudan University, Shanghai 200240， China

　　【Abstract】Objective   To explore the effect of adiponectin on high glucose-induced rat primary pancreatic β-cell apoptosis and the possible mechanism. Method   Rat primary islet cells were isolated and divided into several groups, and β-cell apoptosis and mRNA expressions of some apoptosis-related genes were studied. Result   The β-cell apoptotic rate and expressions of caspase-3 and Bax mRNA were significantly higher in HG group than in NG and HGAPN (HG plus adiponectin) group. Conclusion   Adiponectin can inhibit high glucose-induced primary rat pancreatic β-cell apoptosis and its mechanism might be related to the down regulation of Caspase-3 and Bax mRNA expression.

　　【Key words】Adiponectin;Pancreatic islet cell; Apoptosis