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果糖联合高脂饮食对金黄地鼠糖脂代谢的影响

来自:中国糖尿病资讯网  编辑:songty|点击数:|2011-12-01

 ·糖尿病基础研究·

  叶文慧 孙嘉 蔡德鸿 孙侃 张桦 陈宏 杨力

  基金项目:广东省科技计划项目(2007-Z-005)

  作者单位:510282广州,南方医科大学珠江医院内分泌代谢科

  通讯作者:杨力

  【摘要】 目的 通过果糖或葡萄糖联合高脂饮食喂养叙利亚金黄地鼠,比较不同饮食成分喂养对地鼠糖脂代谢的不同影响。 方法 将金黄地鼠随机分成正常对照组(NC)、高脂组(HF)、果糖高脂组(FRU+HF)、葡萄糖高脂组(Glu+HF),喂养12周,测定体重、血脂、血糖、胰岛素、肝指数,观察肝脏及胰腺形态及组织学变化。 结果 各高脂喂养组地鼠与对照组比较TC、TG、血糖、LDL-C、HDL-C、肝指数升高,胰岛素水平降低,肝脂肪样变,胰岛增生,其中FRU+HF组TG、胰岛素敏感性降低,肝指数升高,肝及胰岛病理变化更明显(P<0.05)。 结论 果糖高脂喂养的金黄地鼠模型特点为TG显著增高、糖耐量减低、胰岛素抵抗及脂肪肝,更适合作为现代人高果糖高脂饮食导致糖脂代谢紊乱的研究模型。

  【关键词】血脂异常;叙利亚金黄地鼠;果糖

  doi:10.3969/j.issn.1006-6187.2011.11.018

  The influence of combined fructose and high-fat diet on the glucose and lipids metabolism of Syrian golden hamster

  YE Wen-hui, SUN Jia, CAI De-hong, et al. Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

  Corresponding auther: YANG Li

  【Abstract】 Objective To study the effect of combined fructose and high-fat diet on the glucose and lipids metabolism. Methods 48 male Syrian golden hamsters were randomized into 4 groups: control group(N), high-fat group(HF), combined fructose and high-fat group(FRU+HF) and glucose plus high-fat group(GLU+HF). Animals had free access to food and water for 12 weeks. Body weight, plasma glucose TG, TC, LDL-C, HDL-C were measured in 0,1st,2nd,6thweek. In 12th week, the insulin level, ISI and hepatic index were determined. The liver and pancreas pathomorphological changes were observed. Results All the high-fat diet groups showed dyslipidemia as compared to control group (P<0.01), FRU+HF group had the dramatically higher plasma TG(P<0.01), and lower ISI. Liver and pancreas pathomorphological changes were more serious. Conclusion All the high-fat diet groups of Syrian hamsters present with dyslipidemia. The FRU+HF group has dramatically higher plasma level of TG, fatty liver, hyperglycemia and insulin resistance, which is more suitable for studying the human metabolism disease caused by high-sugar and high-fat diet.

  【Key words】 Dyslipidemia;Syrian golden hamster;Fructose

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