【摘要】  目的   探讨脂肪酸合成酶（FAS）及其调节因子碳水化合物反应元件结合蛋白（ChREBP）在T1DM小鼠肾脏的表达及胰岛素对其调节的作用。 方法    将C57BL/6小鼠分为对照（Con）组、糖尿病（DM）组和胰岛素治疗（Ins）组。腹腔注射STZ诱导T1DM小鼠模型，观察FAS、ChREBP在其肾脏的表达，以及胰岛素对其的调节。 结果    Con 、DM 、Ins 组TG水平为（0.99±0.466），（2.25±0.66），（1.29±0.24）mmol/L，TC水平为（1.62±0.10）,（1.90±0.12）,（1.68±0.11）mmol/L(P＜0.05)；肾脏FAS及ChREBP蛋白均相对集中表达于肾皮质的肾小管上皮细胞；DM组肾组织中FAS和ChREBP蛋白水平较Con组降低，胰岛素可使糖尿病小鼠肾脏以上2种蛋白含量有所回升；DM组肾脏FAS含量在mRNA水平也较Con组低，下降约50%（P＜0.01）。胰岛素治疗可使糖尿病小鼠肾脏FAS含量上升0.34倍（P＜0.01）；与Con组相比，糖尿病小鼠肾脏ChREBP含量在mRNA水平降低，下降约70%（P＜0.01），胰岛素治疗可使糖尿病小鼠肾脏ChREBP含量上升1.96倍（P＜0.01）。

 结论   糖尿病小鼠肾脏FAS和ChREBP的表达量较非糖尿病小鼠降低，胰岛素可上调糖尿病小鼠肾脏FAS和ChREBP的表达水平。

     【关键词】  糖尿病；1型；脂肪酸合成酶；碳水化合物反应元件结合蛋白

Expression of fatty acid synthase and carbohydrate responsive element binding protein in kidney of type1   diabetic mouse and its regulation by insulin

   【Abstract】 Objective  To investigate the expression of fatty acid synthase (FAS) and its regulatory factor carbohydrate responsive element binding protein (ChREBP), and its regulation by insulin in in kidney of type1 diabetic mouse  Methods  C57BL/6 mouse were divided into three groups: control group (Con), diabetes group (DM) and insulin treatment group (Ins). Diabetic animal model were induced by intraperitoneal streptozotocin injection. The expression of FAS and ChREBP in the kidney were measured. Insulin regulation effects on FAS and ChREBP were also detected in all groups. Results  TG levels were（0.99±0.466）vs（2.25±0.66）vs（1.29±0.24）mmol/L， TC  levels were（1.62±0.10）vs（1.90±0.12）vs（1.68±0.11）mmol/L in Con, DM and Ins groups respectively (P<0.05). FAS and ChREBP protein were abundantly expressed in renal tubular epithelial cells in the cortex of kidney. FAS and ChREBP protein levels were lower in DM group than in Con group. Insulin could increase the protein level of FAS and ChREBP. The mRNA level of FAS in diabetic kidney was reduced by 50% in DM group than in Con group (P<0.01). But insulin treatment could increase 0.34 times higher of the mRNA level of FAS (P＜0.01). Similarly, the mRNA level of ChREBP in diabetic kidney was reduced by 70% in DM group than Con group (P＜0.01）,and use of insulin could upregulate the mRNA level of ChREBP to about 1.96 times higher (P＜0.01）.  Conclusion  FAS and ChREBP expression in diabetic kidney decrease compared with nondiabetic mouse. Insulin can restore the mRNA and protein level of FAS and ChREBP in kidney of diabetic mouse.

【Key words】 Diabetes mellitus；type 1；Fatty acid synthase (FAS)；Carbohydrate responsive element binding protein (ChREBP)