【关键词】  磷脂酰基转移酶1（ALCAT1）；心磷脂；糖尿病；冠心病

The influencing factors and changes of Phospholipid Acyltransferase 1 in myocardial cell of diabetic patients with coronary heart disease  YANG Jun-peng, SHEN Xiao-lei, YAN Shu-fang, et al. Department of Endocrinology, Henan Province People's Hospital, Zhengzhou 450000, China

【Abstract】  Objective  To investigate the mRNA and protein expression level of phospholipid acyltransferase 1 (ALCAT1) in myocardial cell of T2DM patients with coronary heart disease, and analyze the influencing factors.  Methods  A total of 131 patients undergoing cardiac surgery in our hospital were enrolled in this study and divide into 4 groups: cheumatic heart disease group; rheumatic heart disease with T2DM group; coronary heart disease group; coronary heart disease with T2DM group. A tissue of grain size from right auricle was obtained during surgery. mRNA and protein levels of ALCAT1 were tested by RT-PCR and Western blot.  Results  (1) The level of SBP, DBP, TC, LDL-C, HDL-C, TG, FPG, HbA₁c, Scr and ejection fraction (EF) showed significant difference among those groups (P＜0.05）. (2) The mRNA levels of ALCAT1 were also significantly different in those groups (P＜0.05）; And the protein level of ALCAT1 showed similar trend （P＜0.05）. (3) The mRNA level of ALCAT1 was positively correlated with age, SBP, DBP, TC, LDL-C, TG, FBG, HbA₁c and Scr（P＜0.05）, but negatively correlated with HDL-C and EF（P＜0.05）. Conclusion The mRNA and protein levels of ALCAT1 in myocardial cell were higher in coronary heart disease than in rheumatic heart disease, and higher in those patients with diabetes than those non-diabetic patients. Coronary heart disease and diabetes may aggravate the cardiolipin’s pathological reconstruction in myocardial cell, and caused mitochondrial dysfunction.

【Key  Words】  Phospholipid Acyltransferase 1 (ALCAT1); Cardiolipin（CL）; Diabetes mellitus; Coronary heart disease(CHD)