【摘要】 目的 分析非增殖期糖尿病视网膜病变(NPDR)患者血清microRNA(miRNA)表达谱的特征。 方法   选取T2DM患者60例，根据荧光素眼底血管造影结果分为糖尿病无视网膜病变(NDR)组和非增殖期糖尿病视网膜病变(NPDR)组，各30例。采用miRNA微流体芯片技术检测并筛选出两组血清中差异表达的miRNAs，RT-qPCR对部分差异表达基因进行验证。 结果   芯片检测发现，与NDR组比较，NPDR组4个血清miRNAs表达上调，5个血清miRNAs表达下调(P<0.05)。RT-qPCR验证，4个上调的miRNAs，其中，miR-19a、miR-29c、miR-4659b的差异表达有统计学意义(Z＝-4.184、-4.450、-2.794，P<0.05)，且受试者工作特征(ROC)曲线下面积(AUC)分别为0.814、0.834、0.710。 结论    NPDR患者血清miRNA表达谱中有9个miRNAs差异表达明显，其中，表达上调的miR-19a、miR-29c、miR-4659b可作为潜在的NPDR候选生物标志物。
      【关键词】 糖尿病视网膜病变；非增殖期；血清；微小RNA

      【Abstract】  Objective   To explore the characteristics of serum microRNA (miRNA) expression profiles in patients with non-proliferative diabetic retinopathy (NPDR)． Methods    A total of 60 patients with type 2 diabetes (T2DM )were selected and divided into two groups according to their fundus fluorescein angiography results：T2DM without diabetic retinopathy(NDR group，n=30) and T2DM withnon-proliferative diabetic retinopathy (NPDR group，n=30)．MiRNA with different expression in serum
of the two groups were screened and selected by miRNA microarray，in which some were validated by real-time quantitative PCR(RT-qPCR)． Results   There were 4 serum miRNAs with their expression levels up-regulated and 5 serum miRNAs down-regulated in NPDR group as compared with those in NDR group shown by miRNA microarray analysis(P＜0.05)．RT-qPCR validation showed that among 4 up-regulated miRNAs，the difference of miR-19a，miR-29c and miR-4659b expression levels were statistically significant between two groups (Z=-4.184，-4.450，-2.794，P<0.05)，and their AUC of ROC were 0.814， 0.834 and 0.710 respectively． Conclusion    There are 9 serum miRNAs with their expression levels significantly different between two groups．MiR-19a，miR-29c and miR-4659b with up-regulated expression levels may potentially serve as candidate biomarkers of NPDR．
      【Key words】 Diabetic retinopathy(DR)；Non-proliferative(NPDR)；Serum；microRNA(miRNA)