【摘要】 目的  探讨母鼠及断乳后高脂饮食对子代雄鼠(下称“子鼠”)糖脂代谢和肠道菌群的影响。方法   C57BL/6J小鼠在孕期、哺乳期及子鼠断乳后分别持续予高脂饲料或正常饲料，至32周龄观察子鼠糖脂代谢指标及肠道菌群的变化。 结果   子鼠断乳时，高脂饮食(HF)组母鼠较正常饮食(Con)组母鼠体重更重(P<0.05)，而FPG比较，差异无统计学意义。32周龄时，HF组子鼠较Con组子鼠体重更重(P<0.05)，且出现糖耐量异常和IR。HF组子鼠TG和TC水平高，并出现肝脏脂肪变性。肠道菌群分析结果表明，HF组子鼠肠道菌群生物多样性低于Con组子鼠。在门的水平上，HF组子鼠软壁菌门和拟杆菌门比例下降，而变形菌门升高[(1.13±0.09)％vs(6.47±1.85)％](P<0.05)。在属的水平上，粪球菌属、瘤胃球菌属、普氏菌属、乳杆菌属和不可培养属比例均下降，而拟杆菌属比例升高[(7.14±1.58)％"US(24.30±5.28)％](P<0.05)。 结论   母鼠及断乳后高脂饮食能导致子鼠糖脂代谢异常和肠道菌群失调。
     【关键词】高脂饮食；糖脂代谢；肠道菌群；子代；小鼠

     【Abstract】 Objective  To explore the effect of maternal and post-weaning high fat diet on glucose and lipid metabolism and intestinal microbiota in male offspring mice． Methods   C57BL／6J mice were fed with a high fat diet(HF)or a normal diet(Con)during the period of pregnancy and 1actation．Their male offspring were continuously fed with diet of HF or COn until 32 weeks old．Indicators of glucose and lipid metabolism and composition of intestinal microbiota were compared between different groups．Results    The maternal mice were significantly heavier in HF group than in COn group at the time of weaning(P<0.05)．There was no significant difference in the level of fasting blood glucose (FPG)
between HF and Con maternal mice group．At the age of 32 weeks，the offspring in HF group were significantly heavier than those in Con group(P<0.05) and presented with IGT and IR．Plasma TG and TC levels of HF offspring group were relatively high and 1iver steatosis was observed．Analysis on microbiota showed that the biodiversity of intestinal flora was lower in HF group than in Con group．At phylum level，the abundance of tenericutes and bacteroidetes decreased and the abundance of proteobacteria increased significantly in HF group than in Con group (P<0.05)．At genus level．the abundance of lactobacillus，ruminococcus，prevotella，eoproeoceus，and the uncultured decreased and the abundance ofbacteroides increased significantly in HF group than in Con group (P<0.05)． Conclusion Maternal
and post-weaning high fat diet can deteriorate glucose and lipid metabolism and disturb the balance of intestinal microbiota in the offspring．
    【Key words】  High fat diet；Glucose and lipid metabolism；Intestinal microbita；Offspring；Mice