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来自：中国糖尿病杂志 编辑：孙连庆 王煊 李晓瑾等|点击数：|2016-02-26
【摘要】 目的 观察丹参酮Ⅱ A（TanⅡ A）对波动性高糖（IHG）所致的雪旺细胞（SC）凋亡的影响。 方法 原代培养大鼠SC分为正常糖组(Con，5.6 mmol/L)、稳定性高糖组（HG，50 mmol/L）、IHG组（IHG，5.6～50 mmol/L）、渗透压对照组（mannitol）及IHG加不同浓度TanⅡ A(0.1、1、10 μmol/L)组。检测线粒体膜电位(MMP)、细胞凋亡率及凋亡相关蛋白表达。 结果 与Con组和HG组比较，IHG组MMP下降[（0.51±0.02）vs（1.42±0.08）vs（0.66±0.02）]，细胞凋亡率增高[(42.33±2.76)% vs（31.64±3.04）% vs (5.01±0.26)%](P＜0.05或P＜0.01)；线粒体细胞色素c（cyto-c）及凋亡诱导因子（AIF）水平降低，胞浆cyto-c及胞核凋亡诱导因子（AIF）表达增高（P＜0.01）；活化半胱天冬氨酸蛋白酶-9（caspase-9）及caspase-3表达增高（P＜0.01）。 结论 TanⅡ A能抑制SC凋亡，其机制是caspase活性降低，从caspase依赖性及非依赖性途径发挥作用。
【关键词】 丹参酮Ⅱ A；波动性高糖；雪旺细胞；凋亡；凋亡诱导因子
【Abstract】 Objective To investigate the effectinfluence of tanshinone IIA (Tan IIA) on sSchwann cells (SCs) apoptosis induced by intermittent high glucose (IHG). Methods SCs from primary cultured rats were divided into seven groups: SCs with normal glucose of 5.6mmol/L (Con group)，SCs with high glucose of 50mmol/L (HG group), SCs with intermittent high glucose of 5.6-50mmol/L (IHG group), mannitol group, and IHG plus different concentrations of with Tan IIA ( of 0.1 μmol/L, 1 μmol/L, and 10 μmol/L) groups. Mitochondrial transmembrane potential (MMP), apoptosis rate and the expression levels of apoptosis-related proteins were determined in all groups. Results As compared with Con and HG groups, MMP was lower (1.42±0.08 vs 0.66±0.02 vs 0.51±0.02), apoptosis rate was higher (5.01±0.26% vs 31.64±3.04% vs 42.33±2.76%) (P＜0.05 or P＜0.01) in IHG group., The expression levels of cytochrome c (cyto-c) and apoptosis inducing factor (AIF) in mitochondria decreasedwere lower, but the expression levels of cytoplasmic cyto-c and nuclear AIF in cytoplasm and nucleus were higherincreased (P＜0.01) in IHG group., and The expression levels of activated caspase-9 and caspase-3 were higher (p<0.01) in IHG group. Conclusion TanⅡ may reduce the activity of caspase through caspase-dependent and -independent pathway, thereby protect SCs from apoptosis.【Key words】 TanshinoneⅡ A (TanⅡ A); Intermittent high glucose (IHG); Schwann cells(SCs); Apoptosis; Apoptosis inducing factor （AIF）
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