【关键词】  人肾小球内皮细胞；低氧；高糖；多糖-蛋白复合物

 Effect of hypoxia and high glucose on the thickness of polysaccharide-protein complex on human glomerular endothelial cell surface

     【Abstract】  Objective  To investigate the effect of hypoxia and high glucose on the thickness of polysaccharide-protein complex on human glomerular endothelial cell (HRGEC) surface.  Methods  HRGECs by in vitro culture were randomly divided into three groups: hypoxia and high glucose group (n=20), normoxia and high glucose group (n=20) and normal control group (Con, n=20). The thickness of polysaccharide-protein complex on the HRGEC surface among three groups was compared through immunofluorescent staining.  Results  Immunofluorescent staining of polysaccharide-protein complex was strongest in Con group, with a decreased trend in normoxia and high glucose group, followed by hypoxia and high glucose group. The thickness of polysaccharide-protein complex in Con group was higher than in normoxia and high glucose group and in hypoxia and high glucose group [(3.892±0.183) vs (2.913±0.137) vs (2.062±0.152) nm, P＜0.05]. The levels of nitric oxide (NO) secreted by HRGECs cultured for 24 h, 48 h and 72 h were highest in Con group and lowest in hypoxia and high glucose group (P＜0.05).  Conclusion  Hypoxia and high glucose can reduce the thickness of polysaccharide-protein complex on HRGECs surface and inhibit the secretion of NO.

【Key  words】 Human glomerular endothelial cells（HRGECs）; Hypoxia; High glucose; Polysaccharide-protein complexes