【摘要】目的 研究糖基化终末产物(AGEs)对真皮成纤维细胞(Fb)细胞核因子κB(NF-κB)活化的影响及对普罗布考的保护作用。方法 体外分离培养Fb，分为对照(Con)组、AGE-BSA组及普罗布考干预(Probucol)组。水溶性四唑盐(WST)法检测细胞增殖，免疫荧光染色检测NF-κB的活化、实时定量PCR检测MCP-1mRNA表达。结果 与Con组相比，AGE-BSA组细胞增殖能力减退，NF-κB表达增加，细胞MCP-1mRNA表达升高 (P<0.05或P<0.01);与AGE-BSA组相比，Probucol组细胞增殖能力增强，NF-κB表达下降，细胞MCP-1mRNA表达下调(P<0.05或P<0.01)。结论 普罗布考对AGE-BSA诱导的Fb的保护作用可能是通过促进Fb增殖，抑制NF-κB活化及减低MCP-1表达来实现的。

　　【关键词】 普罗布考;糖基化终产物;成纤维细胞;核因子κB

　　【Abstract】 Objective To investigate the influence of advanced glycation end products(AGEs) on the activation of NF-κB in dermal fibroblasts and the protection of probucol induced by. Methods Fibroblasts were isolated and cultured in vitro, and then divided into 3 groups: control group, AGEs group, probucol group. The proliferation of fibroblasts was determined by WST and the activation of NF-κB was observed by immunofluorescence microscope. The mRNA expression of MCP-1 was detected by Real Time PCR. Results Compared with control group, the proliferation of fibroblasts was decreased in AGE-BSA group, but the expression of NF –κB and the mRNA expression of MCP-1 were increased in AGE-BSA group (P<0.05或P <0.01); compared with AGE-BSA group, the proliferation of fibroblasts was increased in probucol group, while the expression of NF –κB and the mRNA expression of MCP-1 were reduced(P <0.05or P <0.01). Conclusion Protective effect of probucol on dysfunction of fibroblasts induced by AGEs may be related with its effect on inhibition of the activation of NF-κB, promotion of the proliferation of fibroblasts and reduced expression of MCP-1.

　　【Key words】 Probucol;Advanced glycation end products;Fibroblasts;NF-κB