【摘要】目的 观察沙格列汀对T2DM大鼠主动脉核因子κB(NF-κB)、血管细胞间黏附因子1(VCAM-1)表达的影响。 方法 将成模大鼠分为糖尿病对照(DM)组、格列本脲治疗(DMG)组和沙格列汀治疗(DMS)组。DMG、DMS组分别予格列本脲、沙格列汀治疗8周。12周末检测各组FPG、FIns、血脂、NF-κB和VCAM-1表达，计算胰岛素抵抗指数(HOMA-IR)，观察大鼠主动脉形态学变化。 结果 12周末，与DMG组比较，DMS组FIns[(11.38±1.56)vs(5.76±0.38)μIU/ml]、TG[(1.17±0.15)vs(0.72±0.25)mmol/L]、LDL-C[(0.57±0.05)vs(0.52±0.03)mmol/L]、HOMA-IR[(12.78±1.53)vs(6.39±0.51)]、NF-κB[(143.33±13.38)vs(121.67±11.43)]和VCAM-1[(153.33±12.74)vs(133.00±11.53)]表达降低(P<0.05)。 结论 沙格列汀在改善糖、脂代谢的同时，可下调糖尿病大鼠主动脉内皮升高的NF-κB、VCAM-1蛋白表达量，可能参与改善大血管并发症。

　　【关键词】 沙格列汀;糖尿病;大鼠;核因子κB;血管细胞间黏附因子1

　　The effect of saxagliptin versus glibenclamide on the expression of nuclear factor κB and vascular cell adhesion molecule 1 in aorta endothelium of type 2 diabetic rats and its clinical significance QI Cai-hui, JIN Yong-jun, YANG Mei-zi, et al. Departmnt of Endocrinology, Yantai Hospital of Binzhou Medical University, Yantai 264100, China

　　Corresponding author：JIN Yong-jun, E-mail：endojin@126.com

　　【Abstract】Objective To investigate the effects of saxagliptin vs glibenclamide on the expression of nuclear factor κB (NF-κB) and vascular cell adhesion molecule 1 (VCAM-1) in aorta endothelium of T2DM rats and the possible mechanism. Methods SD rats were induced into the model of T2DM rats with the method introduced before. Normal SD rats were as NC.Then the diabetic group was treated with Saxagliptin (DMS) and Glibenclamide (DMG) while other diabetic rats were treated with physiological saline (DM) for eight weeks. After that, blood was taken from abdominal aorta for examining biochemical indexes including FPG, FIns, TG, TC, HDL-C and LDL-C. The thoracic aorta was isolated to observe the histological parameters by HE stain. The protein expression of NF-κB and VCAM-1 in aorta endothelium was observed with immunohistochemical method. Rusults At the end of twelfth week, as compared with DMG group, DMS group showed lower levels of FIns [(5.76±0.38)vs(11.38±1.56)μIU/ml], TG [(0.72±0.25)vs(1.17±0.15)mmol/L], LDL-C [(0.52±0.03)vs(0.57±0.05)mmol/L], HOMA-IR [(6.39±0.51)vs(12.78±1.53)], NF-κB[(121.67±11.43)vs(143.33±13.38)] and VCAM-1 [(133.00±11.53)vs(153.33±12.74)] (all P<0.05). Conclusion Saxagliptin is superior to glibenclamide for decreasing the expression of NF-κB and VCAM-1 in aorta endothelium, improving glucolipid metabolism and perhaps decreasing vascular complications in T2DM rats.

　　【Key words】 Saxagliptin;Diabetic mellitus;Rats;Nuclear factor κB (NF-κB);Vascular cell adhesion molecule 1 (VCAM-1)