·糖尿病临床研究·

　　【摘要】 目的 探讨不同阶段T2DM患者循环miRNA-126的水平变化及与内皮型一氧化氮合酶(eNOS)及相关代谢指标的关系。 方法 采用RT-PCR检测血浆miRNA-126表达水平，ELISA测定eNOS水平，分析miRNA-126与eNOS及相关代谢指标的关系。 结果 (1)与健康对照(NC)组比较，血浆miRNA-126在T2DM前期(A)组、单纯T2DM(B)组及T2DM并大血管病变(C)组中均下降(P<0.05)。(2)miRNA-126与LDL-C、DBP及eNOS呈正相关(r=0.224、0.164、0.661，P均<0.05)，与FPG、HDL-C、HbA1c及年龄呈负相关(r=-0.293、-0.153、-0.258、-0.277，P均<0.05)。(3)多元逐步回归分析显示，FPG(β=-0.159，P<0.01)、年龄(β=-0.030，P<0.01)、LDL-C(β=0.208，P=0.037)、eNOS(β=0.353，P<0.01)是影响血浆miRNA-126的相关因素。 结论 循环miRNA-126在不同阶段T2DM患者中均呈低表达,FPG、年龄、LDL-C及eNOS是影响miRNA-126的相关因素，推测miRNA-126可能受糖脂代谢的调控，其下调可能与血管内皮功能障碍有关。

　　【关键词】 糖尿病，2型;miRNA-126;血管病变;糖脂代谢;内皮功能障碍

　　【Abstract】 Objective To investigate the changes in the levels of plasma miRNA-126 in different stages of T2DM patients，and the correlation of miRNA-126 with endothelial nitric oxide synthase( eNOS )and metabolic parameters. Methods The levels of plasma miRNA-126 were measured by real-time PCR (RT-PCR) . The levels of plasma eNOS were measured by enzyme-linked immunosorbent assay(ELISA). The correlation of plasma miR-126 with eNOS and metabolic parameters was also analyzed. Results (1) The levels of plasma miRNA–126 in pre-diabetes (A)group, simple T2DM (B)group and T2DM with macroangiopathy (C)group were significantly lower(P<0.05) compared with healthy control (NC) group. (2) There were positive correlations between miRNA-126 and LDL-C, DBP and eNOS(r=0.224,0.164，0.661, respectively,P<0.05). There were negative correlations between miRNA-126 and FPG, HDL-C，HbA1c,and age(r=-0.293,-0.153,-0.258，-0.277, respectively, P<0.05). (3) Multiple stepwise regression analysis showed that FPG (β=-0.159,P<0.01), age (β=-0.030,P<0.01), LDL-C (β=0.208, P = 0.037) and eNOS(β=0.353，P<0.01)were the correlative factors for plasma miRNA-126 . Conclusion The levels of circulating miRNA-126 are decrease in different stages of T2DM patients. FPG, age, LDL-C and eNOS were the correlative factors for the miRNA-126, Speculating that miRNA-126 may be the control factor to blood glucose and lipid metabolism,the decline of miRNA -126 may be relate to vascular endothelial dysfunction.

　　【Key Words】 Diabetes mellitus，type 2; miRNA-126; Vasculopathy ;Glucose and lipid metabolism; Endothelial dysfunction