·糖尿病基础研究·

　　人工发酵虫草菌丝体干粉对链脲佐菌素诱导糖尿病大鼠心脏NF-κB和TGF-β1表达影响的研究

　　谷优优 高桦 吴梅筠 张鑫 张鹏 赵相卓 马中书 邱明才

　　【摘要】 目的 观察人工发酵虫草菌丝体干粉(CM)对糖尿病大鼠心肌核因子-κB(NF-κB)和转化生长因子-β1(TGF-β1)表达的影响。 方法 健康雄性SD大鼠53只，鼠龄8周，体重(220±20)g，采用数字表法将实验大鼠随机分为正常对照组(n=10)、单纯糖尿病组(n=10)、CM小剂量组[n=12;CM 0.6 g/(kg.d)灌胃]、CM中剂量组[(n=11;CM 2.5 g/(kg.d) )灌胃、CM大剂量组[n=10;CM 5 g/(kg.d)灌胃]。单次尾静脉注射链脲佐菌素(45 mg.kg-1)造模，成模8周后处死大鼠，采用免疫组织化学法、RT-PCR法检测心肌NF-κB 、TGF-β1 蛋白和mRNA的表达水平。结果 单纯糖尿病组心肌NF-κB 、TGF-β1蛋白和mRNA表达均上调(P<0.05=，CM干预可下调NF-κB 、TGF-β1蛋白和mRNA的异常表达(P<0.05=。结论 心肌组织中，NF-κB 和TGF-β1表达的改变可能参与了糖尿病心肌病的发生，CM可能通过调节NF-κB 和TGF-β1在心肌的表达发挥对心肌的保护作用。

　　【关键词】糖尿病;糖尿病心肌病;核因子-κB;转化生长因子-β1;人工发酵虫草菌丝体干粉;大鼠

Effect of artificial fermented cordyceps mycelium on the expressions of NF-κB and TGF-β1 in the cardiac muscle of STZ diabetic rats GU You-you, GAO Hua, WU Mei-jun, et al. Department of Endocrinology, The General Hospital of Tianjin Medical University, Tianjin 300052, China

　Corresponding author：GAO Hua, E-mail: huagao0706@vip.sina.com

　　【Abstract】Objective To observe the effect of artificial fermented cordyceps mycelium (CM) on the expressions of NF-κB and TGF-β1 in the cardiac muscle of streptozocin-induced diabetic rats. Methods A total of 53 healthy male SD rats, aged 8 weeks, weighted 220±20g, were randomized into normal control group (n=10), diabetic group (n=10), and CM group [n=12, 0.6g/(kg.d); n=11, 2.5g/(kg.d); n=10, 5g/(kg.d), for gavage]. Tail vein injection with streptozotocin (45mg.kg-1) was adopted to produce the diabetic rat models. Diabetic rats were sacrificed after 8 weeks. The expressions of NF-κB and TGF-β1 proteins and mRNA in the cardiac muscle were determined by using immunohistochemistry staining and reverse transcription–polymerase chain reaction (RT-PCR) method. The data were analyzed by using one-factor variance analysis. Results The expressions of NF-κB and TGF-β1 proteins and mRNA in the cardiac muscle of diabetic rats were significantly increased (P<0.05). The intervention with cordyceps mycelium could decrease the expressions of NF-κB and TGF-β1 proteins and mRNA (P<0.05). Conclusion The changes in the expressions of NF-κB and TGF-β1 in the myocardial tissue may be involved in the occurrence of diabetic cardiomyopathy. Cordyceps mycelium may play its role on myocardial protection through regulating the expressions of NF-κB and TGF-β1 in the myocardium.

　　【Key words】Diabetes mellitus; Diabetic cardiomyopathy; Nuclear factor-kappa B (NF-κB); Transforming growth factor -βl (TGF-β1); Artificial fermented cordyceps mycelium; Rats