·糖尿病基础研究·

　　陈毅光 薛耀明 关美萍 朱波 沙建平

　　作者单位：510515广州，南方医科大学南方医院内分泌代谢科

　　通讯作者：薛耀明，E-mail: yaomingxue@126.com

　　【摘要】目的探讨波动性高糖下血红素加氧酶1(HO-1)对INS-1细胞氧化应激的影响。 方法以体外培养的INS-1细胞为研究对象，分为正常对照组、持续性高糖组、波动性高糖组、钴原卟啉(CoPP)+波动性高糖组、锌原卟啉(ZnPP)+波动性高糖组。各组均干预72h，Western blot检测HO-1蛋白表达水平，流式细胞仪测定活性氧簇(ROS)水平，四氮唑蓝(MTT)法测定细胞活力。结果波动性高糖组HO-1蛋白表达及ROS水平均显著高于持续性高糖组(P均<0.01)，波动性高糖组细胞活力显著低于持续性高糖组(P<0.05);与波动性高糖组相比，CoPP+波动性高糖组HO-1表达水平及细胞活力升高、ROS水平下降(P均<0.05)，而ZnPP+波动性高糖组呈现相反的变化趋势。结论波动性高糖较持续性高糖引起INS-1细胞产生更严重的氧化应激，在波动性高糖条件下，HO-1可能通过抗氧化应激对INS-1细胞发挥保护效应。

　　【关键词】波动性高糖;血红素加氧酶1;氧化应激;β细胞

　　doi:10.3969/j.issn.1006-6187.2012.02.019

　　Effects of heme oxygenase-1 on the oxidative stress of INS-1 cells under fluctuant high glucoseCHEN Yi-guang, XUE Yao-ming, GUAN Mei-ping, et al. Department of Endocrinology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

　　Corresponding author: XUE Yao-ming,E-mail: yaomingxue@126.com

　　【Abstract】ObjectiveTo study the effects of heme oxygenase-1(HO-1) on the oxidative stress of INS-1 cells under fluctuant high glucose. MethodsINS-1 cells were cultured and divided into five groups: control, persistent high glucose (PHG), fluctuant high glucose (FHG), CoPP + FHG, and ZnPP+ FHG. After 72 hours of intervention, the expressions of HO-1 protein were determined by Western blot, the intracellular reactive oxygen species (ROS) were detected by flow cytometry, and the cell viabilities were evaluated by MTT assay. ResultsThe HO-1 expressions and ROS levels were higher in the FHG group than in the PHG group (P<0.01, respectively). The cell viabilities were significantly lower in the FHG group than in the PHG group (P<0.05). In comparison with FHG, CoPP+ FHG had greater HO-1 protein expressions and cell viabilities, but lower ROS levels (P<0.05, respectively), and the ZnPP+FHG had lower HO-1 protein expressions and cell viabilities, but greater ROS levels (P<0.01, respectively). ConclusionsFluctuant high glucose may trigger more severe oxidative stress in INS-1 cells than persistent high glucose and HO-1 may protect INS-1 cells against oxidative stress under fluctuant high glucose.

　　【Key words】Fluctuant high glucose; Heme oxygenase-1; Oxidative stress;β-cell