糖尿病基础研究

　【摘要】 目的 观察高脂饮食对C57BL/6大鼠出现外周神经病变的影响。方法 将3周龄雌性C57BL/6大鼠随机分为正常对照组(NC)和高脂饮食组，隔周行腹腔内葡萄糖耐量试验，于高脂饮食组小鼠开始出现糖耐量异常时行热痛觉及神经传导速度测定，并检测血清中脂质水平、总超氧化物歧化酶(T-SOD)活性和丙二醛(MDA)含量。结果 分组喂养14周后，高脂饮食组小鼠出现糖耐量异常，热反应时间缩短，运动和感觉神经传导速度均明显减慢(P均<0.05)。高脂饮食组大鼠TC、HDL-C、LDL-C分别较NC组升高86.99%、38.0%、85.42%(P均<0.01)，血清中T-SOD活性及MDA含量均显著升高(P均<0.05)。结论 高脂饮食可诱导C57BL/6大鼠发生外周神经病变，血脂紊乱及氧化应激可能是危险因素。

　　【关键词】 高脂饮食;外周神经病变;血脂紊乱;氧化应激;大鼠

　　Research on high-fat diet induced peripheral neuropathy in C57BL/6 mice TANG Dou, XU Ling-ling, XUE Yao-ming. Department of Endocrinology, Nanfang Hospital Affiliated to Southern Medical University, Guangzhou 510515, China

　　Corresponding author: XUE Yao-ming, Email: xueyaoming999@126.com

　　【Abstract】 Objective To investigate the effect of high-fat diet on peripheral neuropathy in C57BL/6 mice. Methods Female C57BL/6 mice aged of 3 weeks were randomly assigned to the control and high-fat diet (HFD) groups. An intraperitoneal glucose tolerance test was performed every other week. Measurements of thermal pain threshold and nerve conduction velocity (NCV) were taken when the HFD-fed mice displayed glucose intolerance. Lipid profile, total superoxide dismutase (T-SOD) activity and malondialchelyche (MDA) content in serum were examined. Results Following 14 weeks on a high fat diet, mice displayed glucose intolerance and significantly decreased thermal pain threshold, motor nerve conduction velocity (MNCV), and sensory nerve conduction velocity (SNCV) (all P<0.05). The HFD-fed mice showed an increase in serum total cholesterol(TC), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) respectively by 86.99%, 38.0%, and 85.42% (all P<0.01) compared with mice on a control diet, as well as increased T-SOD activity and MDA content significantly (both P<0.05). Conclusion High-fat diet produces peripheral neuropathy in C57BL/6 mice. Dyslipidemia and oxidative stress may be the risk factors.

　　【Key words】High-fat diet; Peripheral neuropathy; Dyslipidemia; Oxidative stress; Rats