·糖尿病分子生物学与遗传学·

刘静婧 任伟 李素芳 郑晓雅 李金超

基金项目：科技部863基金资助项目(2004AA07)

作者单位：400016 重庆，重庆医科大学附属第一医院内分泌科

通讯作者：任伟，E-mail:renwei67@sina.com

【摘要】 目的 探讨重庆地区视黄醇结合蛋白4(RBP4)基因多态性与2型糖尿病(T2DM)及临床表型的关联性。 方法 运用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS) 技术检测中国重庆地区335例无亲缘关系的人群(T2DM患者184例，正常对照151名)中RBP4基因rs17484721和rs3758539多态性。 结果 RBP4基因rs17484721和rs3758539位点多态性在糖尿病和对照组之间差异均无统计学意义(P>0.05)。糖尿病组rs17484721AA基因型携带者的HOMA2IR值明显高于其他基因型(ANCOVA=5.181，P<0.05)，rs3758539GA+AA基因型携带者的空腹血糖值明显高于GG基因型(ANCOVA=6.246，P<0.05)。 结论 中国汉族人群中RBP4基因多态性可能与T2DM相关。

【关键词】 糖尿病，2型;视黄醇结合蛋白4 ;基因多态性

doi:10.3969/j.issn.1006-6187.2011.07.004

The association between the MALDI-TOF-MS detected RBP4 gene polymorphism and type 2 Diabetes mellitus

LIU Jing-jing,REN Wei,LI Su-fang, et al.Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Corresponding author：REN Wei, E-mail: renwei67@sina.com

【Abstract】 Objective To discuss the relationship of retinal binding protein 4(RBP4) gene polymorphism with type 2 diabetes mellitus(T2DM) and clinical phenotype in Chongqing area, China. Method sA technology of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was applied to detect the polymorphism of rs17484721 and rs3758539 locus in RBP4 gene in 184 T2DM patients and 151 normal unrelated controls. Result sThere was no statistically significant difference in genotypes and allele frequency of polymorphism of rs17484721 and rs3758539 locus in RBP4 gene between diabetes and normal control group (P>0.05). In the diabetic group, the value of the HOMA2IR was significantly higher in AA genotype of rs17484721 than in other genotypes(ANCOVA=5.181，P<0.05)，the value of the fasting blood glucose was significantly higher in GA+AA genotype of rs3798539 than in GG genotypes(ANCOVA=6.246，P<0.05). Conclusion sRBP4 gene polymorphism may be associated with type 2 diabetes in Chinese Han population.

【Key words】 Diabetes, type 2; Retinal binding protein 4; Gene polymorphism