·糖尿病基础研究·

　　【摘要】 目的 探讨血管生成素-1(Ang-1)对糖尿病大鼠肾脏病变的影响。 方法 96只雄性SD大鼠随机分为正常对照(NC)组、DN组、Ang-1载体(AV)组和空载体(BV)组。STZ诱导DN模型，8周后尾静脉注射Ang-1-腺病毒载体或腺病毒空载体。检测不同时点血糖、尿蛋白及肾脏病理;采用免疫组织化学、荧光和RT-PCR检测肾组织Ang-1及其受体络氨酸激酶-2(Tie-2)蛋白和mRNA。 结果 AV组24 hUAlb[8周(52.33±15.67)mg/24 h，28周(40.50±7.42)mg/24 h]及血糖[8周(26.28±0.81)mmol/L，28周(17.48±1.14)mmol/L]降低(P<0.05);肾脏病理变化减轻，肾小球面积未减小(P>0.05);肾组织Ang-1、Tie-2 mRNA[8周(76.55±13.21)，28周(90.47±5.04)]及蛋白[8周(140.85±8.45)，28周(150.84±10.48)]升高 (P<0.05)。 结论 外源性Ang-1可上调DN大鼠肾组织Ang-1和Tie-2的表达，缓解DN。

　　【关键词】 糖尿病肾病;促血管生成素-1;腺病毒

　　Protective effects of angiopoietin-1 on kidney of rats with diabetic nephropathy CAO Ming-yan，HU Shi-min，ZHANG Zi-yang，et al. Department of Nephropathy，The Affiliated Hospital，Zunyi Medical College，Zunyi 563003，China

　　Corresponding author：YANG Yi-bin，E-mail：yyb1011@sina.com

　　【Abstract】 Objective To investigate the effect of recombinant adenovirus angiopoietin-1(Ang-1) on renal lesions in diabetic rats. Methods A total of 96 male SD rats were randomly divided into 4 groups：normal control (NC) group，diabetic nephropathy (DN) group，Ang-1-vector (AV) group and blank vector (BV) group. The DN model was induced by injection of streptozotocina. Ang-1-vector and blank vector were injected by tail intravenous injection 8 weeks later. The blood glucose，urine protein，and renal pathology of different time points were detected. Ang-1，Tie-2 protein and its mRNA in renal tissue were tested by RT-PCR，immunochemistry and immunofluorescence respectively. Results The 24 hUAlb[8 weeks(52.33±15.67)mg/24 h，28 weeks(40.50±7.42)mg/24 h] and blood glucose [8 weeks(26.28±0.81)mmol/L，28 weeks(17.48±1.14)mmol/L] were decreased significantly (P<0.05)，renal pathological change was alleviated，and the decrease of glomerular area in Ang-1 treated group was not significant (P>0.05). The expressional of Ang-1 and Tie-2 mRNA [8 weeks (76.55±13.21)，28 weeks (90.47±5.04)]and protein in renal tissue [8 weeks (140.85±8.45)，28 weeks(150.84±10.48)]were increased significantly( P<0.05). Conclusion Exogenous Ang-1 can upregulate the expression of Ang-1 and Tie-2 in renal tissue，and relieve renal lesions in diabetic rats.

　　【Key words】 Diabetic nephropathy(DN);Angiopoietin-1(Ang-1);Adenovirus