·糖尿病基础研究·

　　【摘要】 目的 讨论不同浓度胰升血糖素(Glg)及葡萄糖(Glu)对MIN6胰岛β细胞株胰岛素分泌和细胞增殖活力的影响。 方法 采用不同浓度的Glu(2.8、16.7 mmol/L)及Glg(100、500、1000 ng/L)处理MIN6细胞，ELISA测定细胞上清液中胰岛素的含量，MTT法测定各组细胞增殖活力，收集整理实验数据并进行统计分析。 结果 (1)胰岛素含量：单纯Glu处理后，16.7 mmol/L组高于2.8 mmol/L组(P<0.001);单纯Glg处理后，1000 ng/L组高于100 ng/L组和500 ng/L组(P<0.01或P<0.001);2.8 mmol/L Glu加不同浓度Glg处理后，2.8 mmol/L Glu+1000 ng/L Glg组高于2.8 mmol/L Glu+100 ng/L Glg组和2.8 mmol/L Glu+500 ng/L Glg组(P<0.001)，2.8 mmol/L Glu+500 ng/L Glg组高于2.8 mmol/L Glu+100 ng/L Glg组(P<0.001);16.7 mmol/L Glu加不同浓度Glg处理后，16.7 mmol/L Glu+1000 ng/L Glg组高于16.7 mmol/L Glu+100 ng/L Glg组和16.7 mmol/L Glu+500 ng/L Glg组(P<0.001)，16.7 mmol/L Glu+500 ng/L Glg组高于16.7 mmol/L Glu+100 ng/L Glg组(P<0.001);(2)细胞增殖活力：2.8 mmol/L Glu加不同浓度Glg处理后，各组细胞活力变化比较，差异无统计学意义(P>0.05);生理浓度Glu(5.6 mmol/L)加不同浓度Glg处理后，与无Glg组比较，其他Glg组增高[(0.36±0.06) vs(0.50±0.06)，(0.59±0.10)，(0.54±0.03)ng/L，P<0.05或P<0.01];16.7 mmol/L Glu加不同浓度Glg处理后，与16.7 mmol/L Glu+1000 ng/L Glg组比较，16.7 mmol/L Glu+100 ng/L Glg、16.7 mmol/L Glu+500 ng/L Glg及无Glg组降低[(0.94±0.15) vs (0.66±0.06)，(0.68±0.14)，(0.68±0.03)ng/L，P<0.01]。 结论 Glg可能通过葡萄糖依赖的方式促进MIN6胰岛β细胞株胰岛素分泌，并增加细胞的增殖活力。

　　【关键词】 胰升血糖素;葡萄糖;胰岛素分泌;细胞增殖

　　The study of effect of glucagon on proliferation and insulin secretion of MIN6 cell LI Si-yuan，WU Yuan-yuan，LI Jun，et al.School of Medicine，Shihezi University，Shihezi 832002，China

　　Corresponding author：LI Jun，E-mail：xjlijun@163.com

　　【Abstract】 Objective To explore the influence of glucagon on insulin secretion and cell proliferation of MIN6 cell with different concentrations of glucagon(Glg) and/or glucose(Glu) treatments. Methods MIN6 cells were treated with different concentrations of glucose(2.8 mmol/L-low Glu，16.7 mmol/L-high Glu) and/or glucagon(100 ng/L-low Glg，500 ng/Lmiddle Glg，1000 ng/L-high Glg) Insulin secretion of the cell lines was determined by enzyme linked immune assay. The proliferation viability of MIN6 cell was measured by MTT.The data were analyzed using SPSS 17.0 software. Results (1) The results of insulin secretion levels of MIN6 cells stimulated by different concentrations of glucagon and/or glucose showed：in the groups only treated with glucose，the content of insulin in high glucose group was higher than that in low Glu group(P<0.001);in the groups only treated with glucagon，the content of insulin in high Glg group was higher than that in middle Glg and low Glg group(P<0.01 and P<0.001，respectively);in low glucose groups treated with different concentrations of Glg，the content of insulin in high Glg group was higher than that in middle Glg and low Glg (P<0.001)，and middle Glg group was higher than that in low Glg group(P<0.001);in high glucose groups treated with different concentrations of Glg，the content of insulin in high Glg group was higher than that in middle Glg and low Glg group(P<0.001)，and middle Glg was higher than that in low Glg group (P<0.001).(2) The results of cell proliferation viability of cell lines showed：in low glucose groups (2.8 mmol/L) treated with different concentrations of Glg，there were no significant difference in the viability of cell proliferation (P>0.05);In 5.6 mmol/L glucose groups treated with different concentrations of Glg，the cell proliferation viability of high Glg，middle Glg and low Glg groups were higher than that in group without Glg treatment[(0.36±0.06) vs (0.50±0.06)，(0.59±0.10)，(0.54±0.03)ng/L;P<0.05，P<0.01 and P<0.01，respectively];In 16.7 mmol/L glucose groups treated with different concentrations of Glg，compared with high Glg group，the cell proliferation viability of groups (middle Glg，low Glg，no Glg) was lower [(0.94±0.15) vs (0.66±0.06)，(0.68±0.14)，(0.68±0.03)ng/L;P<0.01]. Conclusion Glucagon may promote insulin secretion and proliferation viability of MIN6 cells by the glucosedependent pattern.

　　【Key words】 Glucagon;Glucose;Insulin secretion;Cell proliferation