【摘要】  目的  观察小檗碱对T2DM地鼠脂肪组织肝X受体（LXR）及其靶基因表达的影响。  方法  以高脂饮食结合小剂量STZ的方法建立IR及T2DM地鼠模型。成模后随机分成正常对照（Con）组、IR组、T2DM组、小檗碱治疗(BBR)组，治疗9周。  结果  与T2DM组相比，BBR组FPG[(6.19±0.84) vs (13.10±0.93) mmol/L]、OGTT 2 hPG[(8.83±1.28) vs (15.49±1.31) mmol/L]、HOMA-IR[(7.40±1.40) vs (14.80±2.10)]降低（P＜0.05）；RT-PCR结果提示，LXRα[(3.29±0.68) vs (-5.56±-1.23)]、LXRβ[(3.09±0.45) vs (-5.49±-0.95)]基因表达上调，并伴随着LXR靶基因表达的改变（P＜0.05）；Western blot结果提示，LXRα[(0.68±0.12) vs (0.11±0.03)]、LXRβ[(0.70±0.14) vs (0.13±0.03)]蛋白表达上调（P＜0.05）。  结论  LXR及其靶基因参与小檗碱治疗T2DM地鼠脂诱性脂肪组织IR的分子机制。

【关键词】  小檗碱；糖尿病地鼠，2型；肝X受体；胰岛素抵抗；脂肪组织

Effect of berberine on liver X receptor expression of adipose tissuein type 2 diabetic hamsters

【Abstract】  Objective  To investigate the effect of berberine on the expression of adipose tissue liver X receptors (LXRs) and their target genes in type 2 diabetic hamsters.  Methods  The insulin-resistant and type 2 diabetic hamster models were induced by high-fat diet and low-dose streptozotocin.The modeled hamsters were randomly divided into 4 groups: normal control group (Con), insulin-resistant group (IR), type 2 diabetic group (T2DM) and berberine-treated type 2 diabetic group (BBR) for nine-week.  Results  Compared with T2DM group, FPG, OGTT 2 hPG and HOMA-IR in BBR group were significantly lower [(13.10 ± 0.93) vs （6.19 ± 0.84） mmol/L; （15.49 ± 1.31） vs（8.83 ± 1.28） mmol/L; （14.80 ±2.10） vs （7.40 ± 1.40），P＜0.05)]. Real-time PCR showed that gene expression of LXRα[(-5.56±-1.23) vs （3.29 ± 0.68）] and LXRβ[(-5.49 ±-0.95) vs （3.09 ± 0.45）] in BBR group significantly increased with changes of LXR target genes expression (P＜0.05). Western blot showed that protein expression of LXRα （0.11±0.03） vs （0.68±0.12） and LXRβ （0.13±0.03） vs （0.70±0.14） in BBR group significantly increased (P＜0.05).  Conclusion  LXR and its target genes are involved in the molecular mechanism of berberine in treatment of adipose tissue insulin resistance in type 2 diabetic hamsters.

【Key words】  Berberine; Diabetic hamster, type 2; Liver X receptors; Insulin resistance（IR）; Adipose tissue