糖尿病分子生物学和遗传学

　　【摘要】 目的 探讨血管紧张素原基因的M235T位点多态性与糖尿病慢性肾脏疾病(CKD)的关系。 方法 选取健康对照(NC)者203名与T2DM患者311例(正常白蛋白尿组158例，微量白蛋白尿组111例，大量白蛋白尿组42例)，采用聚合酶链反应-限制性片断长度多态性技术进行M235T位点多态性检测。 结果 NC组和T2DM组基因型分布MM、MT、TT分别为2.5%、29.6%、68.0%和2.6%、28.3%、69.1%，两组等位基因M、T频率分别为17.2%、82.8%和16.7%、83.3%。4组间基因型分布比较差异无统计学意义，等位基因频率比较差异无统计学意义;进一步按照男女性别各自比较，4组基因型分布比较差异无统计学意义，等位基因频率比较差异无统计学意义。 结论 血管紧张素原基因M235T多态性可能与CKD无相关性。

　　【关键词】糖尿病，2型;糖尿病慢性肾脏疾病;血管紧张素原基因;基因多态性

　　【Abstract】Objective To investigate the relationship between angiotensinogen (AGT) gene M235T polymorphism and its correlation to chronic kidney disease (CKD) in diabetes. Methods A total of 311 T2DM and 203 normal controls (NC) were recruited. The diabetic subjects were further subdivided according to UAlb/Cr and estimated glomerular filtration rate (eGFR). Polymerase chain reaction and restriction fragment length polymorphism were used for M235T genotyping. Results The frequencies of MM, MT, TT genotype were 2.5%, 29.6%, 68.0% in NC group and 2.6%, 28.3%, 69.1% in T2DM group. The frequencies of M, T allele were 17.2%, 82.8% in NC group and 16.7%, 83.3% in T2DM group. No significant difference in genotype or allele frequencies distribution was found among norm-albuminuric, micro-albuminuric, overt-albuminuric cases and controls. The study groups were stratified by gender, still no abnormality in distributions of genotypes and alleles was shown. Conclusion AGT gene M235T polymorphism may not be associated with CKD of T2DM development.

　　【Key words】Diabetes mellitus，type 2;Chronic kidney disease (CKD) in diabetes;Angiotensinogen (AGT) gene;Gene polymorphism