·糖尿病足病·

　　周倩 肖正华 陈定宇 余绮玲 何凌

　　基金项目：广州市科技局应用基础研究项目(2006J1-CO261)

　　作者单位：510180广州市第一人民医院内分泌内科

　　通讯作者：肖正华，E-mail:xzh2998@126.com

　　【摘要】目的研究黄芪多糖(APS)对糖尿病足(DF)截肢患者成纤维细胞(Fb)增殖和胶原合成的影响。方法取非DM创面及DF截肢患者皮肤进行Fb培养后，分为对照组、糖尿病足溃疡(DFUs)组及APS(10、40、160、640μg/ml)组。观察细胞形态学改变、绘制生长曲线、检测Fb增殖及胶原合成。结果与对照组相比，DF截肢患者溃疡处Fb形态改变，数量明显减少，细胞增殖、胶原合成能力减退;APS在10~160μg/ml浓度范围内可增加体外培养的DF截肢患者溃疡处Fb数量，促进细胞增殖和胶原合成，且呈量效、时效依赖关系;过高浓度(≥640μg/ml)的APS对Fb增殖、胶原合成产生抑制作用。结论在一定浓度范围内APS促进DF截肢患者溃疡处Fb增殖和胶原合成，过高浓度APS则产生抑制效应。

　　【关键词】黄芪多糖;糖尿病足;截肢;成纤维细胞;细胞增殖;胶原蛋白

　　doi:10.3969/j.issn.1006-6187.2012.03.008

　　The effect of Astragalus Polysaccharides on biological characteristics of fibroblasts from diabetic foot amputation patientsZHOU Qian,XIAO Zheng-hua,CHEN Ding-yu,et al.Department of Endocrinology,the First Municipal People’s Hospital of Guangzhou,Guangzhou 510180,China

　　Corresponding author: XIAO Zheng-hua, E-mail: xzh2998@126.com

　　【Abstract】ObjectiveTo investigate the influence of Astragalus Polysaccharides(APS) on the proliferation and collagen synthesis of fibroblasts from diabetic foot amputation patients. MethodsFibroblasts were isolated and cultured in vitro, and then exposed to different concentrations of APS (10,40,160,640μg/ml) respectively.The cell morphology was observed and the growth curve was drawed.The proliferation of fibroblasts was determined by MTT and the collagen synthesis was measured with the method of hydroxyproline. ResultsCompared with control group,the proliferation and collagen synthesis responses of fibroblasts derived from DFUs (diabetic foot ulcerations) were decreased in DFU group.APS(10-160μg/ml)groups could promote the responses in a time-dose manner.APS 640μg/ml groups could inhibit the responses. ConclusionAPS could promote the proliferation and collagen synthesis of fibroblasts from diabetic foot amputation patients within a certain range of concentration, but over-high concentration of APS might inhibit the responses.

　　【Key words】Astragalus polysaccharides;Diabetic foot;Amputation;Fibroblasts;Proliferation;Collagen synthesis