磺脲类药物对胰岛β细胞凋亡的影响

·糖尿病基础研究·

王庆美 季虹 黄丽芳 孙海玲 荣海钦

作者单位：250062山东省内分泌与代谢病研究所

通讯作者：荣海钦，E-mail：haiqinrong@126.com

【摘要】 目的 观察第二代和第三代磺脲类药物对胰岛β细胞INS-1凋亡的影响。 方法 采用大鼠胰岛素瘤细胞INS-1作为胰岛β细胞模型，药物干预后观察细胞形态学变化，用MTT和TUNEL法检测细胞增殖、凋亡情况，并测定基础和高糖刺激后胰岛素(Ins)分泌量。 结果 与阴性对照组相比，药物干预细胞4 h，低浓度格列美脲对细胞凋亡无影响(P>0.05)，其他药物组细胞凋亡率明显增加(P<0.05);药物干预1 d及4 d，各药物组细胞凋亡率明显增加(P<0.05)。 结论 磺脲类药物可以促进胰岛β细胞的凋亡，且呈时间-剂量依赖性增加，提示对2型糖尿病(T2DM)患者临床治疗时要合理用药。

doi:12.3969/j.issn.1006-6187.2011.04.019

The effect of sulfonylureas on apoptosis of pancreatic beta cells of INS-1 strain

WANG Qing-mei, JI Hong, HUANG LI-fang，et al. Shandong Institute of Endocrine and Metabolic Diseases, Ji′nan 250062, China

Corresponding author:RONG Hai-qin, E-mail: haiqinrong@126.com

【Abstract】 Objective To study the effect of sulfonylureas on apoptosis of INS-1 cell. Methods INS-1 cells were routinely cultured and then the proliferation and apoptosis of the cells were evaluated by MTT and TUNEL, The insulin secretion was investigated by radioimmunoassay( RIA). Results As compared with negtive control group, after treatment for 4 hours, glimepiride didn’t increase apoptotic cells (P>0.05); while apoptotic rate was increased in other drugs group (P<0.05). After treatment for 1 day, apoptotic cells increased obviously(P<0.05); after treatment for 4 days, apoptotic rate was much higher (P<0.05). Conclusion Sulfonylureas can increase apoptosis of INS-1 cells in a time-and dose-dependent manner.This provides a new thought on studying pancreatic islet cell function and on reasonable use of sulfonylureas in patients with type 2 diabetes mellitus.

【Key words】Sulfonylureas; INS-1 cell; Apoptosis