·糖尿病基础研究·

　　基金项目: 陕西省科技攻关项目(2003K10-G114);西安市科学技术局科技计划项目(SF08002)

　　作者单位：710004西安，西安交通大学医学院第二附属医院内分泌科(王玉环、达婷、张静、吴娟娟、闫丽丽)，干三病区(郑华东)

　　王玉环 达婷 张静 吴娟娟 郑华东 闫丽丽

　　【摘要】 目的 探讨c-Jun氨基末端激酶(JNK)抑制剂SP600125在游离脂肪酸(FFA)诱导的胰岛β细胞凋亡中的作用。 方法 采用MTT法观察FFA对小鼠βTc3细胞生长的抑制作用，流式细胞术观察细胞凋亡率，免疫细胞化学和Western blot法检测p-JNK、p-c-Jun在FFA作用下及SP600125阻断JNK信号通路情况下的表达。 结果 FFA可诱导体外生长的βTc3细胞凋亡，与对照组比较明显增加(P<0.05)。FFA诱导βTc3细胞凋亡过程中，p-JNK和p-c-Jun蛋白显著增加(P<0.05);用SP600125预处理βTc3细胞后， p-JNK和p-c-Jun蛋白含量较未处理组明显减少(P<0.05)。 结论 FFA可诱导小鼠βTc3细胞凋亡，凋亡过程通过JNK途径实现。

　　【关键词】游离脂肪酸;βTc3细胞;c-Jun氨基末端激酶;凋亡

　　doi:10.3969/j.issn.1006-6187.2011.01.017

　　Effects of JNK inhibitor SP600125 on free fatty acids-induced apoptosis in mouse β Tc3 cells

　　WANG Yu-huan，DA Ting，ZHANG Jing, et al. Department of Endocrinology, the Second Affiliated Hospital, Medical School of Xi′an Jiaotong University, Xi′an 710004，China

　　【Abstract】 Objective To investigate the anti-apoptotic effects of JNK inhibitor SP600125 on apoptosis in mouse βTc3 cells. Methods MTT assay was used to observe the inhibitory effects of FFA on generation of βTc3 cells .The apoptotic rate of the cells was detected by flow cytometry. The protein expressions of p-JNK, p-c-Jun were detected by immunocytochemistry and Western blot. Results The growth of the βTc3 cells were obviously inhibited by FFA in vitro, and significant apoptosis was present. FFA induced activation of p-JNK, p-c-Jun. As a JNK inhibitor, SP600125 suppressed FFA -induced activation of p-JNK and p-c-Jun. Conclusion FFA can inhibit the proliferation of mouse βTc3 cells and induce βTC3 cells apoptosis. The JNK signaling pathway is involved in the mechanism of FFA-induced apoptosis.

　　【Key words】Free fatty acid; βTc3 cells; c-Jun amino-terminal kinase; Apoptosis