糖尿病临床研究

　　【摘要】目的 探讨人巨细胞病毒(HCMV)、柯萨奇B组病毒(CVB)及ICA、GADAb、FBG检测多因素与糖尿病的关系。 方法 选取我院2011~2013年住院或门诊糖尿病患者100例，其中，T1DM(T1DM组)、T2DM(T2DM组)患者各50例。另选取健康对照(NC组)者50名。检测各组ICA、GADAb、人巨细胞病毒lgM抗体(HCMV-lgM)、人巨细胞病毒IgG抗体(HCMV-IgG)、柯萨奇B组病毒IgM抗体(CVB-IgM)及FBG水平。 结果 抗HCMV-IgM、抗HCMV-IgG、抗CVB-IgM、ICA、GADA阳性例数(阳性率)T1DM组分别为16(32%)、3(6%)、1(2%)、36(72%)、21(42%)，T2DM组分别为11(22%)、22(44%)、4(8%)、1(2%)、 29(58%)，NC组分贝为12(24%)、1(2%)、13(26%)、1(2%)、0(0%)(P<0.05)。与NC组比较，T2DM组抗HCMV-IgG、ICA抗体检测阳性结果差异有统计学意义(P<0.05)。3组FBG水平比较差异有统计学意义(P<0.05)。 结论 联合检测HCMV、CVB抗体及ICA、GADAb、FBG对糖尿病患者的诊疗具有重要价值。

　　【关键词】糖尿病，1型;糖尿病，2型;人巨细胞病毒;柯萨奇B组病毒

　　The combined detection of HCMV, CVB antibody,ICA, GADA and FBG in patients with Type 1 and Type 2 DM ZHAO Chang-xin ,WEN Jiang-tao, LI Shi-bao ，et al.Department of Laboratory，Lianyungang City Second People's Hospital，Lianyungang 222002，China

　　[Abstract] Objective To explore the diagnostic significance of combined detection of HCMV, CVB antibody,ICA, GADA and FBG in patients with Type 1 and Type 2 DM. Methods A total of 100 inpatients or outpatients with diabetes mellitus admitted to our hospitalfrom 2011 to2013 were selected as study subjects. 50 patients in T1DM group and 50 patients in T2DM group and 50 healthy people as normal control were selected in the study. ICA was detected with indirect immunofluorescence method, GADAb,HCMV-lgM, HCMV- IgG, CVB-IgMwere determined with ELISA method and FPG was determined with AU5800 biochemicalanalyzer. ResultsThe positive cases number(positive rate) in type 1, type 2 DM group and healthy control group were 16 (32%), 3 (6%), 1 (2%)for anti-HCMV-IgM; 36 (72%) , 21 (42%), 11 (22%)for anti-HCMV-IgG; 22 (44%), 4 (8%), 1 (2%)for anti-CVB-IgM; 29 (58%), 12 (24%) and 1 (2%)for ICA; 13 (26%), 1 (2%) and 0 (0%)GADA, (all p<0.05 )respectively. Comparison between T2DM group and healthy control group, the difference inpositiveresults of anti-HCMV-IgG and ICA antibody were statistically significant(P<0.05). There were statistical significances for FBG among the three groups,(P<0.05). Conclusion Joint detection of HCMV, CVB antibody, ICA, GADAb, and FPG is of great value to the diagnosis and treatment T1DM and T2DM.