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糖尿病大鼠肾脏8-羟基脱氧鸟苷和超氧化物歧化酶表达的变化及西格列汀对其影响的研究

来自:中国糖尿病杂志  编辑:陈洋 王建平 付婷 姜爱花 邓珊珊|点击数:|2015-01-28

  【摘要】 目的 探讨西格列汀对糖尿病大鼠肾脏8-羟基脱氧鸟苷(8-OHdG)和超氧化物歧化酶(SOD)表达的影响。 方法 复制糖尿病大鼠模型,随机分为西格列汀1 mg/(kg·d)干预组(Sita1,n=15),西格列汀10 mg/(kg·d)干预组(Sita2,n=15),糖尿病未干预组(DM,n=16)及正常对照组(NC,n=15)。14周末观察各组FPG、FIns、肾功能、24 h尿蛋白、8-OHdG和SOD的变化。 结果 与NC组比较,DM、Sita1和Sita2组FIns及血清SOD活性均明显下降(P<0.05),FPG、BUN、SCr、24 h尿蛋白及尿8-OHdG均明显升高 (P<0.05)。与DM组比较,Sita1组BUN、SCr、24 h尿蛋白及尿8-OHdG均明显降低(P<0.05),血清SOD活性明显升高(P<0.05);Sita2组FPG、BUN、Scr、24h尿蛋白及24h尿8-OHdG明显降低(P<0.05),FIns和血清SOD活性明显升高(P<0.05)。 结论 氧化应激参与糖尿病大鼠肾脏病变的发生发展,西格列汀对糖尿病大鼠肾脏有一定的保护作用。

  【关键词】 糖尿病;糖尿病肾病;西格列汀;8-羟基脱氧鸟苷;超氧化物歧化酶

  【Abstract】 Objective To investigate the effect of sitagliptin on the expressions of 8-OHdG and SOD in diabetic rats kidney. Methods The rat models of diabetes were randomly divided into the following four groups: Sita1 group (Sitagliptin with the dose of 1 mg/kg body weight daily, n=15), Sita2 group (Sitagliptin with the dose of 10 mg/kg body weight daily, n=15), DM group (untreated diabetes group, n=16), and the NC group(n=15). 14 weeks later, The levels of FPG, FIns, BUN, Scr, urinary protein, 8-OHdG and SOD were detected. Results Compared with NC group, FIns and serum SOD significantly decreased in DM, Sita1 and Sita2 group (P<0.05), while FPG, BUN, Scr, urinary protein for 24 h and urinary 8-OHdG excretion significantly increased in these group (P<0.05). Compared with DM group, BUN, Scr, urinary protein for 24 h and urinary 8-OHdG excretion significantly decreased in Sita1 group (P<0.05), while serum SOD significantly increase in this group (P<0.05); FPG, BUN, Scr, urinary protein for 24 h and urinary 8-OHdG excretion significantly decreased in Sita2 group(P<0.05), in which FIns and SOD significantly increased(P<0.05). Conclusion Oxidative stress may take part in the pathogenesis of diabetic kidney injury, and Sitagliptin has some renoprotective effect on diabetic rats.

  【Key words】 Diabetes mellitus;Diabetic nephropathy;Sitagliptin;8-hydroxy-2'-deoxyguanosine(8-OHdG);Superoxide Dismutase(SOD)

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